Document Detail

Developmental regulation of expression of the lactate dehydrogenase (LDH) multigene family during mouse spermatogenesis.
MedLine Citation:
PMID:  2401207     Owner:  NLM     Status:  MEDLINE    
Expression of the Lactate Dehydrogenase (LDH) genes during various stages of spermatogenesis was studied by using a combination of Northern blot analyses and in situ hybridization techniques. These studies have indicated that developmentally programmed expression of all three functional LDH genes occurs during differentiation of germ cells. The LDH/C (ldh-3) gene was expressed exclusively during meiosis and spermiogenesis, beginning in leptotene/zygotene spermatocytes and continuing through to the elongated spermatids. LDH/C (ldh-3) gene expression was accompanied by transient expression of the LDH/A (ldh-1) gene in pachytene spermatocytes and round spermatids. The LDH/B (ldh-2) gene was expressed mainly in Sertoli and spermatogonial cells. By using somatic cell hybrids, the LDH/C (ldh-3) gene has been mapped to mouse chromosome 7, establishing that it is syntenic with the LDH/A (ldh-1) gene locus. Experimental observations made in this study provide new insight into the order and sequence of events involved in the regulation of gene expression of the LDH gene family during spermatogenesis.
K Thomas; J Del Mazo; P Eversole; A Bellvé; Y Hiraoka; S S Li; M Simon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  109     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-10-24     Completed Date:  1990-10-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  483-93     Citation Subset:  IM    
Division of Biology, California Institute of Technology, Pasadena 91125.
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MeSH Terms
Blotting, Northern
Gene Expression Regulation / physiology*
L-Lactate Dehydrogenase / genetics*
Meiosis / genetics
Mice, Inbred Strains
Nucleic Acid Hybridization
Seminiferous Epithelium / analysis
Spermatogenesis / genetics*
Transcription Factors / analysis
Grant Support
Reg. No./Substance:
0/Transcription Factors; EC Dehydrogenase

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