| Developmental regulation of chromatin composition during mouse embryogenesis: somatic histone H1 is first detectable at the 4-cell stage. | |
| | |
MedLine Citation:
|
PMID: 1425354 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have examined the distribution of histone H1 in oocytes and preimplantation embryos of the mouse, using a polyclonal antibody raised against the histone H1 subtypes present in somatic cells. Immunofluorescence and immunoblotting analyses failed to detect somatic histone H1 in germinal vesicle (GV)-stage oocytes. In contrast, somatic histone H1 was detectable by immunofluorescence in the nuclei of GV oocytes previously injected with histone H1 as well as the nuclei of ovarian granulosa cells, and by immunoblotting in 8-cell embryos. 1- and 2-cell embryos examined by immunofluorescence did not contain detectable somatic histone H1. At the early 4-cell stage (54-56 hours post-hCG), 5 of 52 embryos contained somatic histone H1 in one or more nuclei. By the late 4-cell stage (66-68 hours post-hCG), however, 58 of 62 embryos contained somatic histone H1. In 8-cell embryos, morulae and blastocysts, all nuclei contained somatic histone H1 in every case. When embryos were exposed to the transcriptional inhibitor, alpha-amanitin, beginning at the late 2-cell stage, they cleaved to the 4-cell stage but fewer than 10% developed histone H1 immunoreactivity. When treatment began at the early 4-cell stage, the embryos that remained at the 4-cell stage in the presence of the drug developed histone H1 immunoreactivity in half of the cases. Embryos that reached the 5- to 8-cell stage in the presence of the drug developed histone H1 immunoreactivity in every case.(ABSTRACT TRUNCATED AT 250 WORDS) |
| | |
Authors:
|
H J Clarke; C Oblin; M Bustin |
Related Documents
:
|
19922864 - Nuclei take a position: managing nuclear location. 21273084 - Melampomagnolide b: a new antileukemic sesquiterpene. 6593094 - Regulation of nucleosomal core histone variant levels in differentiating murine erythro... 11766874 - The nuclear envelope: emerging roles in development and disease. 23110004 - Cytologic findings of clear cell adenocarcinoma of the urethra: a case report. 17608804 - Human cdc45 is a proliferation-associated antigen. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Development (Cambridge, England) Volume: 115 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 1992 Jul |
Date Detail:
|
Created Date: 1992-12-03 Completed Date: 1992-12-03 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8701744 Medline TA: Development Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 791-9 Citation Subset: IM |
Affiliation:
|
Department of Obstetrics & Gynecology, McGill University, Montreal, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blastocyst / chemistry* Chromatin / chemistry* Fluorescent Antibody Technique Histones / chemistry* Immunoblotting Mice Morphogenesis / physiology Oocytes / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Chromatin; 0/Histones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ectopic sensory neurons in mutant cockroaches compete with normal cells for central targets.
Next Document: orthodenticle activity is required for the development of medial structures in the larval and adult ...