| Developmental regulation of 14-3-3 epsilon isoform in rat heart. | |
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MedLine Citation:
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PMID: 9443075 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human heart cDNA sequencing yielded a cDNA clone that is similar in DNA and amino acid sequences to that of mouse 14-3-3 epsilon isoform. The 6xHis-tagged H1433 epsilon recombinant protein was expressed in Escherichia coli and its size was approximately 30 kDa. From Northern blot results with human multiple tissues, human skeletal muscle was found to have the highest level of h1433 epsilon mRNA expression, whereas Northern blots of human cancer cell lines detected the highest mRNA level of h1433 epsilon in colorectal adenocarcinoma SW480. The protein expression level of h1433 epsilon and Raf-1 is found to be regulated coordinately during rat heart development, and their protein expression was highest from 14.5 to 16.5 days postcoitum. |
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Authors:
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S C Luk; S M Ngai; S K Tsui; K K Chan; K P Fung; C Y Lee; M M Waye |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 68 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-03-13 Completed Date: 1998-03-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 195-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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14-3-3 Proteins Animals Blotting, Northern Blotting, Western Cloning, Molecular DNA, Complementary / analysis Escherichia coli / genetics Gene Expression / genetics Gene Expression Regulation, Developmental Heart / embryology* Humans Myocardium / chemistry, metabolism* Proteins / genetics* RNA, Messenger / analysis, genetics Rats Tyrosine 3-Monooxygenase* |
| Chemical | |
Reg. No./Substance:
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0/14-3-3 Proteins; 0/DNA, Complementary; 0/Proteins; 0/RNA, Messenger; EC 1.14.16.2/Tyrosine 3-Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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