Document Detail


Developmental profiles of PERIOD and DOUBLETIME in Drosophila melanogaster ovary.
MedLine Citation:
PMID:  19223210     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The clock protein PERIOD (PER) displays circadian cycles of accumulation, phosphorylation, nuclear translocation and degradation in Drosophila melanogaster clock cells. One exception to this pattern is in follicular cells enclosing previtellogenic ovarian egg chambers. In these cells, PER remains high and cytoplasmic at all times of day. Genetic evidence suggest that PER and its clock partner TIMELESS (TIM) interact in these cells, yet, they do not translocate to the nucleus. Here, we investigated the levels and subcellular localization of PER in older vitellogenic follicles. Cytoplasmic PER levels decreased in the follicular cells at the onset of vitellogenesis (stage 9). Interestingly, PER was observed in the nuclei of some follicular cells at this stage. PER signal disappeared in more advanced (stage 10) vitellogenic follicles. Since the phosphorylation state of PER is critical for the progression of circadian cycle, we investigated the status of PER phosphorylation in the ovary and the expression patterns of DOUBLETIME (DBT), a kinase known to affect PER in the clock cells. DBT was absent in previtellogenic follicular cells, but present in the cytoplasm of some stage 9 follicular cells. DBT was not distributed uniformly but was present in patches of adjacent cells, in a pattern resembling PER distribution at the same stage. Our data suggest that the absence of dbt expression in the follicular cells of previtellogenic egg chambers may be related to stable and cytoplasmic expression of PER in these cells. Onset of dbt expression in vitellogenic follicles coincides with nuclear localization of PER protein.
Authors:
Joanna Kotwica; Maureen K Larson; Piotr Bebas; Jadwiga M Giebultowicz
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-02-15
Journal Detail:
Title:  Journal of insect physiology     Volume:  55     ISSN:  1879-1611     ISO Abbreviation:  J. Insect Physiol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-11     Completed Date:  2009-06-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985080R     Medline TA:  J Insect Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  419-25     Citation Subset:  IM    
Affiliation:
Department of Zoology, Oregon State University, 3029 Cordley Hall, Corvallis, OR 97331, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Casein Kinase Iepsilon / genetics,  metabolism*
Drosophila Proteins / genetics,  metabolism*
Drosophila melanogaster / genetics,  growth & development*,  metabolism*
Female
Gene Expression Regulation, Developmental*
Nuclear Proteins / genetics,  metabolism*
Ovarian Follicle / growth & development,  metabolism
Ovary / growth & development,  metabolism
Period Circadian Proteins
Phosphorylation
Protein Transport
Grant Support
ID/Acronym/Agency:
5R01GM073792-03/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Nuclear Proteins; 0/PER protein, Drosophila; 0/Period Circadian Proteins; 0/dco protein, Drosophila; EC 2.7.11.1/Casein Kinase Iepsilon

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