Document Detail


Developmental pattern of gene-specific DNA methylation in the mouse embryo and germ line.
MedLine Citation:
PMID:  1577268     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Methylation patterns of specific genes have been studied by polymerase chain reaction and found to undergo dynamic changes in the germ line and early embryo. Some CpG sites are methylated in sperm DNA and unmodified in mature oocytes, indicating that the parental genomes have differential methylation profiles. These differences, however, are erased by a series of early embryonic demethylation and postblastula remodification events, which serve to reestablish the basic adult methylation pattern prior to organogenesis. During gametogenesis, all of these sites are unmethylated in primordial germ cells but eventually become remodified by 18.5 days postcoitum in both males and females. The final methylation profile of the mature germ cells is then formed by a multistep process of site-specific demethylation events. These results form a basis for the understanding of the biochemical mechanisms and role of DNA methylation in embryonic development.
Authors:
T Kafri; M Ariel; M Brandeis; R Shemer; L Urven; J McCarrey; H Cedar; A Razin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  6     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-06-11     Completed Date:  1992-06-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  705-14     Citation Subset:  IM    
Affiliation:
Department of Cellular Biochemistry, Hebrew University Medical School, Jerusalem, Israel.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA / metabolism*
Dinucleoside Phosphates / metabolism
Embryo, Mammalian / metabolism
Embryonic and Fetal Development / genetics*
Female
Genes / genetics
Male
Methylation
Mice
Molecular Sequence Data
Oocytes / metabolism
Oogenesis / genetics*
Polymerase Chain Reaction
Spermatogenesis / genetics*
Spermatozoa / metabolism
Chemical
Reg. No./Substance:
0/Dinucleoside Phosphates; 2382-65-2/cytidylyl-3'-5'-guanosine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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