| Developmental localization of noradrenergic innervation to the rat cerebellum following neonatal 6-hydroxydopa and morphine treatment. | |
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MedLine Citation:
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PMID: 6813097 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to demonstrate the influence of morphine on the developmental localization of regenerated noradrenergic fibers in rat cerebellum, a glyoxylic histofluorescent method and radiometric assay for norepinephrine (NE) were utilized. An initial reduction of NE in the cerebellum after 6-hydroxydopa [6-OHDOPA; 60 micrograms/g intraperitoneally (i.p.)] was followed by a return to control levels at 3 days, and an elevation above control levels at 7 days. The initial rates of recovery of NE in the cerebellum of the 6-OHDOPA group of rats and the group receiving morphine (20 micrograms/g i.p.) in combination with 6-OHDOPA were identical up to 7 days. However, by 14 days NE content was further elevated in the cerebellum of the morphine +6-OHDOPA group. Histofluorescent microscopic observations of the cerebellar cortex correlated with the biochemical findings. A reduction in cerebellar NE content at 3 days was associated with a reduction in the number of visible histofluorescent fibers in the cerebellar cortex. By 7 days the relative number of fibers in the 6-OHDOPA groups was similar to that seen in the control group, but by 9 days the relative number of fluorescent fibers in the cerebellar cortex was increased above control. By 13 days there was a further increase in the relative number of fluorescent fibers in the cerebellar cortex of the morphine +6-OHDOPA group, as compared to the group treated with 6-OHDOPA alone. These findings provide an anatomic correlate for recovery of noradrenergic fibers after 6-OHDOPA, and demonstrate an action of morphine in enhancing regenerative sprouting. |
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Authors:
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C T Harston; M B Clark; J C Hardin; R M Kostrzewa |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Developmental neuroscience Volume: 5 ISSN: 0378-5866 ISO Abbreviation: Dev. Neurosci. Publication Date: 1982 Mar-Jun |
Date Detail:
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Created Date: 1982-12-18 Completed Date: 1982-12-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7809375 Medline TA: Dev Neurosci Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 252-62 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging* Animals Cerebellar Cortex / drug effects Cerebellum / drug effects* Dihydroxyphenylalanine / analogs & derivatives*, pharmacology Microscopy, Fluorescence Morphine / pharmacology* Nerve Fibers / drug effects Norepinephrine / metabolism* Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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NS 14797/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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21373-30-8/6-hydroxydopa; 51-41-2/Norepinephrine; 57-27-2/Morphine; 63-84-3/Dihydroxyphenylalanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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