Document Detail


Developmental genetics of the essential Drosophila nucleoporin nup154: allelic differences due to an outward-directed promoter in the P-element 3' end.
MedLine Citation:
PMID:  10511559     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Drosophila nup154 encodes a predicted nucleoporin homologous to yeast Nup170p, Nup157p, and vertebrate Nup155, all of which are major components of the nuclear pore complex (NPC). Unlike its yeast homologs, nup154 is essential for viability. Animals with strong loss-of-function nup154 mutations caused by P-element insertion in the 5'-UTR of the gene died as larvae with small discs, brains, and testes. nup154 mRNA expression appeared developmentally regulated in tissues of wild-type embryos, larvae, and adults, suggesting that new nup154 synthesis is required when assembly of new NPCs is required, as in proliferating or growing tissues. Two additional nup154 alleles also associated with different P-element inserts in the 5'-UTR were viable but had strong loss-of-function sterile phenotypes, including failure to maintain spermatogenic stem cells and failure to progress into vitellogenic stages of oogenesis. Lethality vs. viability correlated with orientation of the P-element inserts in the different alleles. Transcript analysis by 5'-RACE suggested a mechanism for allelic differences: an outward-directed promoter internal to the P-element 3' end able to drive sufficient expression of the nup154 transcript for viability but not for fertility.
Authors:
A A Kiger; S Gigliotti; M T Fuller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  153     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-12-06     Completed Date:  1999-12-06     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  799-812     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Base Sequence
Conserved Sequence
Crosses, Genetic
DNA Transposable Elements*
Drosophila Proteins*
Drosophila melanogaster / embryology,  genetics*,  growth & development
Embryo, Nonmammalian / physiology
Female
Gene Expression Regulation, Developmental*
Genes, Lethal
Homozygote
Larva
Male
Molecular Sequence Data
Nuclear Pore Complex Proteins*
Nuclear Proteins / genetics*
Promoter Regions, Genetic*
RNA, Messenger / genetics
Restriction Mapping
Transcription, Genetic*
Translocation, Genetic
Grant Support
ID/Acronym/Agency:
P01-DK53074/DK/NIDDK NIH HHS; R01 GM078176/GM/NIGMS NIH HHS; R01-GM56500/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA Transposable Elements; 0/Drosophila Proteins; 0/Nuclear Pore Complex Proteins; 0/Nuclear Proteins; 0/Nup154 protein, Drosophila; 0/RNA, Messenger
Comments/Corrections

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