Document Detail


Developmental and functional evidence of nuclear immaturity in prepubertal oocytes.
MedLine Citation:
PMID:  16790612     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The nuclear compartment has been proposed as responsible for the developmental arrest of prepubertal mouse oocytes while the studies on prepubertal sheep and cow oocyte model suggested the cytoplasm immaturity accounts for this failure. METHODS: The apparent disagreement on the causes of developmental defects between these two species prompted us to study: (i) follicular and oocyte growth allometry in lambs, (ii) oocyte compartment (nucleus versus cytoplasm) responsible for developmental failure by nucleus exchange between lamb and adult sheep oocytes, (iii) nucleolar features of prepubertal oocytes by ultrastructural observation and (iv) in vivo developmental survey of prepubertally derived embryos. RESULTS: The oocyte growth inside the follicle is asynchronous during prepuberty. The nuclear transfer revealed that the lamb nucleus was responsible for developmental failure. Immature fibrillogranular structure of the nucleolus has been revealed in small lamb oocytes and also in a few adult-size lamb oocytes. Studies in vivo revealed a high occurrence of developmental arrest of prepubertal derived fetuses, which we have attributed to the low genome-wide methylation detected in prepubertal oocytes. CONCLUSIONS: Our studies have indicated incomplete nuclear maturation of prepubertal gamete. The implication of this finding suggests caution when the strategy of rescue of prepubertal oocytes for assisted fertilization is considered such as in the case of therapeutic treatment which precludes the maintenance of fertility of sexually immature patients.
Authors:
Grazyna Ptak; Kazutsugu Matsukawa; Chiara Palmieri; Leonardo Della Salda; Pier Augusto Scapolo; Pasqualino Loi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-21
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  21     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-21     Completed Date:  2007-01-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  2228-37     Citation Subset:  IM    
Affiliation:
Department of Comparative Biomedical Sciences, University of Teramo, Teramo, Italy. gptak@unite.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleolus / metabolism
Cell Nucleus / metabolism
Coculture Techniques
Cytoplasm / metabolism
DNA Methylation
Female
Fertilization in Vitro
Fluorescent Antibody Technique, Indirect
Methylation
Microscopy, Electron, Transmission
Oocytes / metabolism*,  ultrastructure*
Pregnancy
Pregnancy, Animal
Sheep
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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