| Developmental expression of spontaneous activity in the spinal cord of postnatal opossums, Monodelphis domestica: an anatomical study. | |
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MedLine Citation:
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PMID: 19501058 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using Sulforhodamine-101 (SR101) labeling and calcium imaging on in vitro preparations, we investigated the development of spontaneous activity in the spinal enlargements of a marsupial born more immature than eutherian mammals, the opossum Monodelphis domestica. Following the retrograde transport of Calcium Green dye from the limb nerves, we observed the occurrence of spontaneous calcium waves activating the motor columns of the cervical enlargement of opossums aged from P3 to P15 (day of birth: P0) and of the lumbar enlargement from at least P6 to P12. In other preparations, SR101 was added to the bath to identify the active cells. In P1 opossums, only a few SR101-labeled cells were observed in the cervical enlargement and none in the lumbar enlargement. At P5, their number increased cervically and they appeared in the lumbar enlargement. Motoneurons were the major cell type labeled by SR101 but dye leakage made their quantification inaccurate. SR101-labeled cells also occurred elsewhere in the ventral and dorsal grey. Their number increased until P12-14 in both enlargements and then decreased to disappear by P21, the last age examined. Thus in contrast to eutherian mammals, in which spontaneous activity is mostly prenatal, spontaneous activity occurs predominantly postnatally in opossums. It increases at the time when connections from the brain begin to impinge on spinal neurons and when the limbs, especially the hindlimbs, start moving and then decreases as the systems mature. |
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Authors:
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Annie Lavallée; Jean-François Pflieger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-06 |
Journal Detail:
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Title: Brain research Volume: 1282 ISSN: 1872-6240 ISO Abbreviation: Brain Res. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-13 Completed Date: 2009-10-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-9 Citation Subset: IM |
Affiliation:
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Département de Sciences biologiques, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, Québec, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials
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physiology Aging / physiology Animals Animals, Newborn Calcium / metabolism Calcium Signaling / physiology Cell Differentiation / physiology Coloring Agents Extremities / innervation* Marsupialia / anatomy & histology, growth & development Monodelphis / anatomy & histology, growth & development* Motor Neurons / cytology, metabolism Nerve Net / cytology, growth & development* Neurons / cytology, metabolism* Organic Chemicals Rhodamines Species Specificity Spinal Cord / cytology, growth & development* Staining and Labeling / methods |
| Chemical | |
Reg. No./Substance:
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0/Coloring Agents; 0/Organic Chemicals; 0/Rhodamines; 138067-55-7/calcium green; 60311-02-6/sulforhodamine 101; 7440-70-2/Calcium |
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