Document Detail


Developmental expression of intermediate filament proteins in the chick embryo retina: in vivo and in vitro comparison.
MedLine Citation:
PMID:  2673838     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An immunocytochemical study was performed on the chick embryo retina to detect the changes in intermediate filament (IF) composition in correlation with development. Results showed that all three major neurofilament subunits (NF L, M and H) were expressed from very immature neurons at the same time. No delayed expression of NF-H was observed. Vimentin (VT) was also present from very early stages in both neuronal and glial cells, but it was not detectable at hatching. Its distribution changed in retinal layers throughout development. It co-existed with NF in neurons, and conversely, it preceded the appearance of the typical glial fibrillar acidic protein (GFA-P), which was detected in radial fibres of Müller cells from day 16 of incubation. These in vivo data were compared with immunocytochemical staining of retinal cultures, grown either on collagen or on polylysine. NF-L and GFA-P were not detectable in vitro. Moreover, the early expression of NF subunits was shown to be influenced by the type of substratum. The contemporary presence of vimentin and neurofilaments was clearly shown both in neuronal cell bodies and processes. The results also show that in the chick retina the pattern of composition of intermediate filaments changes with maturation. Moreover, in vitro conditions influence the expression of IF components. These data are of interest when performing in vitro analysis of the effect of exogenous substances affecting neuronal maturation and differentiation.
Authors:
S Torelli; V Sogos; M A Marzilli; M D'Atri; F Gremo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental biology     Volume:  48     ISSN:  0176-8638     ISO Abbreviation:  Exp Biol     Publication Date:  1989  
Date Detail:
Created Date:  1989-10-26     Completed Date:  1989-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503247     Medline TA:  Exp Biol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  187-96     Citation Subset:  IM    
Affiliation:
Department of Cytomorphology, School of Medicine, Cagliari, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chick Embryo
Culture Techniques
Fluorescent Antibody Technique
Glial Fibrillary Acidic Protein / metabolism
Immunohistochemistry
Intermediate Filament Proteins / metabolism*
Neurofilament Proteins*
Neuroglia / metabolism
Neurons / metabolism
Retina / embryology*,  metabolism
Vimentin / metabolism
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Intermediate Filament Proteins; 0/Neurofilament Proteins; 0/Vimentin; 0/neurofilament protein L; 108688-71-7/neurofilament protein H; 111365-29-8/neurofilament protein M

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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