Document Detail


Developmental expression and distribution of amphibian glutathione transferases.
MedLine Citation:
PMID:  11287125     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This work is aimed at detecting the expression and location of embryonic Bufo bufo GST (bbGSTP1-1) and adult B. bufo GST (bbGSTP2-2) during toad development, in order to assign a putative role to these enzymes also on the basis of their compartmentalization and to verify whether during the premetamorphic liver ontogeny the bbGSTP2-2 form appears. This study was also performed in the adult liver (the primary site of Pi class GST expression) and in the mature ovary, to discern if the embryonic form derives from maternal form. The results show that the embryos and the ovary express only bbGSTP1-1. Moreover, bbGSTP1-1 distribution is the same both in the early embryos and in the ovary: this strongly suggests that bbGSTP1-1 is of maternal origin. As development goes on, a wide distribution of bbGSTP1-1 all over the differentiating organs is observed. The embryonic liver expresses exclusively the bbGSTP1-1 form, while the adult liver is highly positive only towards the bbGSTP2-2 form. This implies that the switch towards the adult bbGSTP2-2 form occurs in metamorphic or postmetamorphic phases and that the detoxication metabolic requirements of the embryo may be completely fulfilled by the bbGSTP1-1 isoenzyme.
Authors:
F Amicarelli; A M Ragnelli; P Aimola; F Cattani; A Bonfigli; O Zarivi; M Miranda; C Di Ilio
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1526     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-04-05     Completed Date:  2001-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  77-85     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bufo bufo / embryology,  growth & development,  metabolism*
Female
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Glutathione Transferase / metabolism*
Immunoblotting
Immunohistochemistry
Isoenzymes / analysis,  metabolism
Liver / enzymology
Ovary / enzymology
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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