| Developmental expression of EphB6 in the thymus: lessons from EphB6 knockout mice. | |
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MedLine Citation:
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PMID: 12379224 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A member of the largest family of receptor protein kinases, EphB6, lacks its intrinsic kinase activity, but it is expressed in normal human tissues. To investigate the physiological function of EphB6, we generated EphB6 deficient mice. EphB6(-/-) mice developed normally, revealed no abnormality in general appearance, and were fertile. Although a developmental increase of EphB6 in the fetal thymus was confirmed, T-cell development in various lymphoid organs of EphB6(-/-) mice was comparable to those of EphB6(+/+). Even in fetal thymus organ cultures, any developmental differences of EphB6(-/-) and EphB6(+/+) thymocytes were undetectable. The different binding characteristics to ephrin-Fc proteins between EphB6(-/-) and EphB6(+/+) thymocytes demonstrated that ephrin-B2 is the unique ligand for EphB6 among eight known ephrins. While EphB6 was a dominant receptor that binds to ephrin-B2 in adult thymocytes, fetal ones also expressed another EphB that binds to ephrin-B2. Overlapping expression of the EphB subfamily in the fetal thymus might compensate for the loss of EphB6 during the thymic development. |
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Authors:
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Manabu Shimoyama; Hiroshi Matsuoka; Aki Nagata; Nobuko Iwata; Akira Tamekane; Atsuo Okamura; Hiroshi Gomyo; Mitsuhiro Ito; Kou-ichi Jishage; Nobuo Kamada; Hiroshi Suzuki; Tetsuo Tetsuo Noda; Toshimitsu Matsui |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 298 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-10-15 Completed Date: 2002-11-22 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 87-94 Citation Subset: IM |
Affiliation:
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Division of Hematology/Oncology, Department of Medicine, Kobe University School of Medicine, Chuo-ku, Kobe, Hyogo 650-0017, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Cell Count CD4-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / immunology Cell Differentiation Gene Targeting Mice Mice, Knockout Organ Culture Techniques RNA, Messenger / analysis Receptor, EphB6 / genetics*, metabolism* Receptors, Eph Family / metabolism Spleen / immunology Thymus Gland / embryology*, immunology |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Receptor, EphB6; EC 2.7.10.1/Receptors, Eph Family |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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