Document Detail


Developmental competence of transported in-vitro matured macaque oocytes.
MedLine Citation:
PMID:  16454934     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study examines in-vitro maturation (IVM) in a non-human primate model, Macaca fascicularis. The animals had hormonal injections and laparoscopic oocyte retrieval (OR)) at 12- and 24- h after human chorionic gonadotrophin (HCG). The immature oocytes were placed in tightly capped tubes containing pre-equilibrated IVM medium and transported for 5 h in a dry portable 37 degrees C incubator without CO2 supplement. Meiotic spindle was observed at 36-38- h post-HCG by polarized microscopy in 72 and 84.5% of mature oocytes collected at 12- and 24- h post-HCG oocyte retrieval intervals respectively. However, abnormal spindle formations were detected in some IVM oocytes by confocal microscopy. The IVM oocytes were also randomly selected for (i) intracytoplasmic injection with frozen-thawed epididymal M. fascicularis spermatozoa and (ii) nuclear transfer (NT) with fresh M. fascicularis cumulus cells. Embryonic development of sperm-injected embryos was not affected by the 12- and 24- h post-HCG oocyte retrieval intervals (22.5 versus 27.9% respectively). However, embryonic development of NT embryos was significantly affected by the 12- h post-HCG oocyte retrieval interval (4.5 versus 31.7% respectively; P < 0.01). In conclusion, IVM of monkey oocytes in a dry portable incubator for 5 h did not affect the maturation rate. However, the ability of primate oocytes to develop after somatic cell nuclear transfer was affected by oocyte retrieval time post-HCG.
Authors:
Naiqing Chen; Swee-Lian Liow; Ramli Bin Abdullah; Wan Khadijah Wan Embong; Wan-Yue Yip; Lay-Geok Tan; Guo-Qing Tong; Soon-Chye Ng
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  12     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-02-03     Completed Date:  2006-09-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  50-9     Citation Subset:  IM    
Affiliation:
Embryonics International, Gleneagles Hospital, Annex Block, #01-38, 6A Napier Road, Singapore 258500, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods*
Chorionic Gonadotropin
Female
Macaca fascicularis*
Microscopy, Confocal
Mitotic Spindle Apparatus / ultrastructure
Oocytes / cytology*
Specimen Handling / methods*
Sperm Injections, Intracytoplasmic*
Time Factors
Zygote / growth & development*
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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