Document Detail

Developmental changes of tetrahydrobiopterin in rat and human erythrocytes.
MedLine Citation:
PMID:  8926533     Owner:  NLM     Status:  MEDLINE    
In the present study, we investigated the developmental changes of (1) plasma and erythrocyte tetrahydrobiopterin (BH4); (2) erythrocyte GTP cyclohydrolase (the rate-limiting enzyme of BH4 biosynthesis); (3) the permeability of erythrocyte membrane to BH4; and (4) plasma phenylalanine, both in healthy human subjects and Wistar rats. In vitro experiments demonstrated passive transport of BH4 into erythrocytes. In humans, BH4 levels as well as the other parameters were fairly consistent across all age groups. In contrast, Wistar rats showed significant developmental changes in erythrocyte BH4, which were not simply correlated to either GTP cyclohydrolase, permeability to BH4 or plasma phenylalanine levels. This may suggest the existence of other factors regulating the homeostasis of BH4, such as BH4-binding capacity in plasma and/or erythrocytes. These species/age differences in erythrocyte characteristics may influence the pharmacological behavior and clinical efficacy of BH4 in humans and experimental animals.
K Azumagawa; S Suzuki; M Mino
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of nutritional science and vitaminology     Volume:  41     ISSN:  0301-4800     ISO Abbreviation:  J. Nutr. Sci. Vitaminol.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-11-04     Completed Date:  1996-11-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0402640     Medline TA:  J Nutr Sci Vitaminol (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  607-17     Citation Subset:  IM    
Department of Pediatrics, Osaka Medical College, Takatsuki, Japan.
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MeSH Terms
Aging / blood*
Biopterin / analogs & derivatives*,  blood
Cell Membrane Permeability
Child, Preschool
Erythrocyte Membrane / metabolism
Erythrocytes / metabolism*
GTP Cyclohydrolase / blood
Infant, Newborn
Rats, Wistar
Reg. No./Substance:
17528-72-2/5,6,7,8-tetrahydrobiopterin; 22150-76-1/Biopterin; EC Cyclohydrolase

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