| Developmental changes in rat liver mitochondrial populations analyzed by flow cytometry. | |
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MedLine Citation:
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PMID: 1426036 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isolated rat liver mitochondria were split into three density fractions when applied to a Percoll gradient. This phenomenon is observable in the fetus, in the early newborn (1 h), in the suckling newborn (7 days), and in the adult, suggesting that the three density fractions coexist regardless of the state of development. The medium-density fraction sharply decreased immediately after delivery, being replaced by the high-density fraction. Flow cytometry analysis of mitochondrial density fractions stained with rhodamine 123 showed the occurrence in each density fraction and in all developmental states studied of two distinct mitochondrial populations with different fluorescence intensities. Our results suggest that the high-fluorescence population might be an immature form of mitochondria that decreases with the progression of development, coinciding with the postnatal enhancement of mitochondrial respiratory efficiency. |
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Authors:
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C López-Mediavilla; A Orfao; J San Miguel; J M Medina |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 203 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1992-12-04 Completed Date: 1992-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 134-40 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica y Biologia Molecular, Facultad de Farmacia, Universidad de Salamanca, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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physiology* Animals Animals, Newborn Animals, Suckling Cell Fractionation Female Fetus Flow Cytometry Fluorescent Dyes Liver / embryology, growth & development*, ultrastructure Mitochondria, Liver / ultrastructure* Pregnancy Rats Rats, Wistar Rhodamine 123 Rhodamines |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Rhodamines; 62669-70-9/Rhodamine 123 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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