Document Detail


Developmental changes in the long form leptin receptor and related neuropeptide gene expression in the pig brain.
MedLine Citation:
PMID:  11369586     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hypothalamus is the key site of central regulation of energy homeostasis, appetite, and reproduction. The long form leptin receptor (Ob-Rl) is localized within the hypothalamus along with several neuropeptides that are involved in regulation of the neuroendocrine axis. In the present study, developmental changes in gene expression of the Ob-Rl, preproorexin, proopiomelanocortin (POMC), corticotropin releasing factor (CRF), somatostatin, and GnRH in the hypothalamus was studied. Expression of Ob-Rl and neuropeptide mRNA was examined by semiquantitative reverse transcription-polymerase chain reaction in hypothalami collected from 106-day-old fetus (n = 3) and 7-day-old (n = 3), 3.5-mo-old (n = 3), and 6-mo-old (n = 2) gilts. In addition, leptin mRNA expression in the first three ages was examined in back fat. Leptin mRNA expression increased (P < 0.05) by 7 days postnatal, but Ob-Rl mRNA expression increased (P < 0.01) by 3.5 mo. Expression of preproorexin (P < 0.05), somatostatin, and GnRH (P < 0.01) mRNA peaked by 3.5 mo of age while POMC mRNA expression increased markedly (P < 0.01) by 6 mo of age. The CRF mRNA expression did not change across ages. These findings suggest a possible relationship among Ob-Rl and a number of hypothalamic and peripheral peptides in the development of the neuroendocrine axis. These peptides may serve as messengers that link mechanisms that regulate reproduction and energy balance.
Authors:
J Lin; C Richard Barb; R R Kraeling; G B Rampacek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  64     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-22     Completed Date:  2001-08-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1614-8     Citation Subset:  IM    
Affiliation:
Department of Animal and Dairy Science, University of Georgia, Athens, Georgia 30602, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / embryology*,  metabolism
Carrier Proteins / analysis,  genetics*
Corticotropin-Releasing Hormone / genetics
Female
Gene Expression*
Gestational Age
Gonadotropin-Releasing Hormone / genetics
Hypothalamus / chemistry,  metabolism
Intracellular Signaling Peptides and Proteins*
Leptin / genetics
Neuropeptides / genetics*
Pregnancy
Pro-Opiomelanocortin / genetics
Protein Precursors / genetics
RNA, Messenger / analysis
Receptors, Cell Surface*
Receptors, G-Protein-Coupled
Receptors, Leptin
Receptors, Neuropeptide
Reverse Transcriptase Polymerase Chain Reaction
Somatostatin / genetics
Swine / embryology*
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Leptin; 0/Neuropeptides; 0/Protein Precursors; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/Receptors, G-Protein-Coupled; 0/Receptors, Leptin; 0/Receptors, Neuropeptide; 0/orexin receptors; 0/orexins; 33515-09-2/Gonadotropin-Releasing Hormone; 51110-01-1/Somatostatin; 66796-54-1/Pro-Opiomelanocortin; 9015-71-8/Corticotropin-Releasing Hormone

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