|Developmental changes in cellular and extracellular structural macromolecules in the secondary palate and in the nasal cavity of the mouse.|
|PMID: 20572855 Owner: NLM Status: MEDLINE|
|The aim of this study was to analyse the hitherto largely unknown expression patterns of some specific cellular and extracellular molecules during palate and nasal cavity development. We showed that epithelia of the developing palate and the vomerine epithelium express similar sets of structural proteins. With the exception of keratin 15, which becomes barely detectable in the elevated palatal shelves, nearly all of these proteins become upregulated at the presumptive areas of fusion and in the adhering epithelia of the palate and nasal septum. In vivo and in vitro analyses indicated that reduction in the amount of keratin 15 protein is independent of Tgfbeta-Alk5 signalling. Foxa1 expression also highlighted the regionalization of the palatal and nasal epithelia. Owing to the lack of reliable markers of the palatal periderm, the fate of peridermal cells has been controversial. We identified LewisX/stage-specific embryonic antigen-1 as a specific peridermal marker, and showed that numerous peridermal cells remain trapped in the medial epithelial seam (MES). The fate of these cells is probably apoptosis together with the rest of the MES cells, as we provided strong evidence for this event. Heparan sulphate, chondroitin-6-sulphate, and versican displayed dynamically changing distribution patterns. The hitherto-unknown innervation pattern of the developing palate was revealed. These findings may be of value for unravelling the pathogenesis of palatal clefting.|
|Forugh Vaziri Sani; Vesa Kaartinen; Maha El Shahawy; Anders Linde; Amel Gritli-Linde|
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|Type: Journal Article; Research Support, Non-U.S. Gov't|
|Title: European journal of oral sciences Volume: 118 ISSN: 1600-0722 ISO Abbreviation: Eur. J. Oral Sci. Publication Date: 2010 Jun|
|Created Date: 2010-06-24 Completed Date: 2010-09-28 Revised Date: 2014-04-08|
Medline Journal Info:
|Nlm Unique ID: 9504563 Medline TA: Eur J Oral Sci Country: Denmark|
|Languages: eng Pagination: 221-36 Citation Subset: D; IM|
|APA/MLA Format Download EndNote Download BibTex|
Antigens, CD15 / analysis
Apoptosis / physiology
Cell Adhesion / physiology
Chondroitin Sulfates / analysis
Cytoskeletal Proteins / analysis*
Epithelium / embryology
Extracellular Matrix Proteins / analysis*
Heparitin Sulfate / analysis
Hepatocyte Nuclear Factor 3-alpha / analysis
Keratin-14 / analysis
Keratin-15 / analysis
Keratin-6 / analysis
Keratin-8 / analysis
Keratins / analysis
Lamin Type A / analysis
Myosin Heavy Chains / analysis
Nasal Cavity / cytology, embryology*
Nonmuscle Myosin Type IIA / analysis
Palate / cytology, embryology*, innervation
Protein-Serine-Threonine Kinases / analysis
Receptors, Transforming Growth Factor beta / analysis
Signal Transduction / physiology
Transforming Growth Factor beta3 / analysis
Versicans / analysis
Vomer / cytology, embryology
|0/Antigens, CD15; 0/Cspg2 protein, mouse; 0/Cytoskeletal Proteins; 0/Extracellular Matrix Proteins; 0/Foxa1 protein, mouse; 0/Hepatocyte Nuclear Factor 3-alpha; 0/KRT1-17 protein, mouse; 0/Keratin-14; 0/Keratin-15; 0/Keratin-6; 0/Keratin-8; 0/Krt1-14 protein, mouse; 0/Krt1-15 protein, mouse; 0/Krt8 protein, mouse; 0/Lamin Type A; 0/Macromolecular Substances; 0/Receptors, Transforming Growth Factor beta; 0/Tgfb3 protein, mouse; 0/Transforming Growth Factor beta3; 126968-45-4/Versicans; 68238-35-7/Keratins; 9007-28-7/Chondroitin Sulfates; 9050-30-0/Heparitin Sulfate; EC 220.127.116.11/TGF-beta type I receptor; EC 18.104.22.168/Protein-Serine-Threonine Kinases; EC 3.6.1.-/Nonmuscle Myosin Type IIA; EC 22.214.171.124/Myosin Heavy Chains|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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