Document Detail


Developmental changes in cellular and extracellular structural macromolecules in the secondary palate and in the nasal cavity of the mouse.
MedLine Citation:
PMID:  20572855     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to analyse the hitherto largely unknown expression patterns of some specific cellular and extracellular molecules during palate and nasal cavity development. We showed that epithelia of the developing palate and the vomerine epithelium express similar sets of structural proteins. With the exception of keratin 15, which becomes barely detectable in the elevated palatal shelves, nearly all of these proteins become upregulated at the presumptive areas of fusion and in the adhering epithelia of the palate and nasal septum. In vivo and in vitro analyses indicated that reduction in the amount of keratin 15 protein is independent of Tgfbeta-Alk5 signalling. Foxa1 expression also highlighted the regionalization of the palatal and nasal epithelia. Owing to the lack of reliable markers of the palatal periderm, the fate of peridermal cells has been controversial. We identified LewisX/stage-specific embryonic antigen-1 as a specific peridermal marker, and showed that numerous peridermal cells remain trapped in the medial epithelial seam (MES). The fate of these cells is probably apoptosis together with the rest of the MES cells, as we provided strong evidence for this event. Heparan sulphate, chondroitin-6-sulphate, and versican displayed dynamically changing distribution patterns. The hitherto-unknown innervation pattern of the developing palate was revealed. These findings may be of value for unravelling the pathogenesis of palatal clefting.
Authors:
Forugh Vaziri Sani; Vesa Kaartinen; Maha El Shahawy; Anders Linde; Amel Gritli-Linde
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of oral sciences     Volume:  118     ISSN:  1600-0722     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-24     Completed Date:  2010-09-28     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  221-36     Citation Subset:  D; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD15 / analysis
Apoptosis / physiology
Cell Adhesion / physiology
Chondroitin Sulfates / analysis
Cytoskeletal Proteins / analysis*
Epithelium / embryology
Extracellular Matrix Proteins / analysis*
Gestational Age
Heparitin Sulfate / analysis
Hepatocyte Nuclear Factor 3-alpha / analysis
Keratin-14 / analysis
Keratin-15 / analysis
Keratin-6 / analysis
Keratin-8 / analysis
Keratins / analysis
Lamin Type A / analysis
Macromolecular Substances
Mice
Myosin Heavy Chains / analysis
Nasal Cavity / cytology,  embryology*
Nonmuscle Myosin Type IIA / analysis
Palate / cytology,  embryology*,  innervation
Protein-Serine-Threonine Kinases / analysis
Receptors, Transforming Growth Factor beta / analysis
Signal Transduction / physiology
Transforming Growth Factor beta3 / analysis
Up-Regulation
Versicans / analysis
Vomer / cytology,  embryology
Chemical
Reg. No./Substance:
0/Antigens, CD15; 0/Cspg2 protein, mouse; 0/Cytoskeletal Proteins; 0/Extracellular Matrix Proteins; 0/Foxa1 protein, mouse; 0/Hepatocyte Nuclear Factor 3-alpha; 0/KRT1-17 protein, mouse; 0/Keratin-14; 0/Keratin-15; 0/Keratin-6; 0/Keratin-8; 0/Krt1-14 protein, mouse; 0/Krt1-15 protein, mouse; 0/Krt8 protein, mouse; 0/Lamin Type A; 0/Macromolecular Substances; 0/Receptors, Transforming Growth Factor beta; 0/Tgfb3 protein, mouse; 0/Transforming Growth Factor beta3; 126968-45-4/Versicans; 68238-35-7/Keratins; 9007-28-7/Chondroitin Sulfates; 9050-30-0/Heparitin Sulfate; EC 2.7.1.11/TGF-beta type I receptor; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 3.6.1.-/Nonmuscle Myosin Type IIA; EC 3.6.4.1/Myosin Heavy Chains

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