Document Detail

Developmental changes in NO bioavailability in fetal erythrocytes.
MedLine Citation:
PMID:  15565792     Owner:  NLM     Status:  MEDLINE    
S-nitrosohemoglobin (HbSNO), where hemoglobin (Hb) is nitrosated at Cysbeta93, presumably controls delivery of the vasorelaxant nitric oxide (NO) to hypoxic tissues in an oxygen-sensitive manner. Little is known about how Hb regulates NO bioavailability during fetal development. A study was planned to determine the levels of HbSNO and HbFe(II)NO (NO bound to FeII of heme) in the cord blood of newborn infants of different gestational ages and establish their relationship with the levels of fetal Hb (HbF). Blood samples were collected from umbilical cord obtained from normal newborns between 24 and 41 weeks of gestation. Determinations of HbSNO and HbFe(II)NO were performed using chemiluminescence. The proportion of HbF was determined by HPLC. There were 11 preterm (24-34 weeks of gestation) and 11 term infants (37-41 weeks of gestation). The levels of HbSNO varied from 0.37 to 1.72 x 10(-5) mol/mol heme. There was a significant correlation with gestational age (r2 = 0.46, P = 0.0005) due to the effect of the decrease in the amount of HbF (r2 = 0.81, P < 0.0001). The relationship of HbFe(II)NO was not affected by gestational age or the level of HbF (mean 1.68+/-1.15 x 10(-5) mol/mol heme). Under physiological in utero conditions, fetal erythrocytes have lower levels of HbSNO, which increase in the later stage of fetal development. The levels of HbSNO in the fetal red cell are dependent on the level of adult Hb (HbA). The low HbSNO levels at physiological fetal O2 saturations during early development could protect the fetal circulation from an excess release of NO and O2.
Harry Bard; Ann M English; Karine Bellemin; Carmen Gagnon
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Seminars in perinatology     Volume:  28     ISSN:  0146-0005     ISO Abbreviation:  Semin. Perinatol.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-11-29     Completed Date:  2005-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7801132     Medline TA:  Semin Perinatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  312-6     Citation Subset:  IM    
Pediatric Department, University of Montreal, St Justine's Hospital Montreal, Quebec, Canada.
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MeSH Terms
Biological Availability
Case-Control Studies
Cells, Cultured
Erythrocytes / metabolism*
Fetal Blood / cytology*
Fetal Hemoglobin / analysis*
Gestational Age
Hemoglobins / metabolism*
Infant, Newborn
Infant, Premature*
Linear Models
Nitric Oxide / blood*
Oxygen Consumption
Sampling Studies
Term Birth
Reg. No./Substance:
0/Hemoglobins; 0/S-nitrosohemoglobin; 10102-43-9/Nitric Oxide; 9034-63-3/Fetal Hemoglobin

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