| Developmental changes of gangliosides of the rat stomach. Appearance of a blood group B-active ganglioside. | |
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MedLine Citation:
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PMID: 3680254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat stomach gangliosides were purified and their distribution in the different tissue compartments was established. Three major monosialogangliosides were found: GM3, GM1, and a ganglioheptaosylceramide carrying a blood group B determinant. This latter structure was characterized by exoglycosidase degradation, immunostaining with a monoclonal anti-blood group B antibody on thin layer chromatogram, permethylation analysis, electron-impact mass spectrometry of the permethylated-reduced and trimethylsilylated molecule, and 1H NMR spectroscopy of the native ganglioside. It was found to be (Formula: see text) i.e. a GM1 structure substituted with the blood group B determinant and was called B-GM1. A similar structure has been previously identified in precancerous rat liver and chemically induced rat hepatoma (Holmes, E. H., and Hakomori, S. (1982) J. Biol. Chem. 257, 7698-7703). Fucosyl-GM1 was also detected as a minor ganglioside in rat gastric mucosa. The ganglioside profile was modified during the postnatal development. The contribution of GM3 and GD3, which accounted for 95% of the ganglioside sialic acid at birth, decreased during the first 3 weeks of life. GM1, fucosyl-GM1, and B-GM1 were not detected at birth. The concentration of the fucogangliosides increased during the 2nd and 3rd weeks after birth, was stable during the 4th week and then decreased, whereas that of GM1 increased steadily between 6 days and 2 months of age. B-GM1, which has been defined as a tumor-associated ganglioside in the rat liver, was found to be a developmentally regulated antigen of the normal rat stomach. |
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Authors:
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J F Bouhours; D Bouhours; G C Hansson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 262 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1987 Dec |
Date Detail:
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Created Date: 1988-01-12 Completed Date: 1988-01-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 16370-5 Citation Subset: IM |
Affiliation:
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Unité 76 de l'Institut National de la Santé et de la Recherche Médicale, Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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ABO Blood-Group System* Animals Carbohydrate Conformation Chromatography, Thin Layer G(M1) Ganglioside / metabolism G(M3) Ganglioside / metabolism Gangliosides / metabolism* Glycoside Hydrolases / metabolism Magnetic Resonance Spectroscopy Mass Spectrometry Rats Stomach / growth & development*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/ABO Blood-Group System; 0/G(M3) Ganglioside; 0/Gangliosides; 37758-47-7/G(M1) Ganglioside; EC 3.2.1.-/Glycoside Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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