Document Detail

Developmental and cell cycle regulation of alfalfa nucMs1, a plant homolog of the yeast Nsr1 and mammalian nucleolin.
MedLine Citation:
PMID:  8721748     Owner:  NLM     Status:  MEDLINE    
We report here the isolation and characterization of the nucMs1 alfalfa cDNA, whose predicted amino acid sequence structurally resembles the yeast Nsr1 protein and animal nucleolins. These proteins consist of an N-terminal acidic domain, centrally located RNA recognition motifs (RRMs), and a C-terminal glycine- and arginine-rich domain. In comparison with animal nucleolins that contain four RRMs, NucMs1 more closely resembles the yeast Nsr1 protein, which contains only two RRMs. A NucMs1 C-terminal peptide antibody specifically recognized a 95-kD nucleolar protein in alfalfa cells that changed its localization in a cell cycle-dependent manner. The nucMs1 transcript and p95nucMs1 protein levels correlated with cell proliferation, and nucMs1 gene expression was found to be induced in the G1 phase upon mitogenic stimulation of G0-arrested leaf cells. In situ hybridization analysis of different alfalfa organs during various developmental stages showed that nucMs1 gene expression is highest in root meristematic cells, but it is also found in other meristematic cells of the plant body. nucMs1 expression is tightly linked to cell proliferation but does not depend on a particular cell cycle phase. No nucMs1 expression was observed in cells that had exited the cell cycle and were undergoing differentiation or polar growth, indicating that nucMs1 may not be necessary for processes other than cell proliferation.
L Bögre; C Jonak; M Mink; I Meskiene; J Traas; D T Ha; I Swoboda; C Plank; E Wagner; E Heberle-Bors; H Hirt
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant cell     Volume:  8     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-10-09     Completed Date:  1996-10-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  417-28     Citation Subset:  IM    
Institute of Microbiology and Genetics, University of Vienna, Austria.
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MeSH Terms
Base Sequence
Cell Cycle / physiology*
Cell Cycle Proteins / biosynthesis,  metabolism*
Cell Division
Cell Nucleolus / physiology
Fungal Proteins / metabolism*
Gene Library
Medicago sativa / genetics,  physiology*
Molecular Sequence Data
Nuclear Proteins / biosynthesis,  metabolism*
Phosphoproteins / biosynthesis,  metabolism*
Plant Proteins / biosynthesis,  metabolism*
RNA-Binding Proteins*
Saccharomyces cerevisiae / genetics,  physiology*
Saccharomyces cerevisiae Proteins*
Transcription, Genetic
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Fungal Proteins; 0/NSR1 protein, S cerevisiae; 0/NuM1 protein, Medicago sativa; 0/Nuclear Proteins; 0/Oligodeoxyribonucleotides; 0/Phosphoproteins; 0/Plant Proteins; 0/RNA-Binding Proteins; 0/Saccharomyces cerevisiae Proteins; 0/nucleolin

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