Document Detail

Developmental and cell cycle progression defects in Drosophila hybrid males.
MedLine Citation:
PMID:  17947412     Owner:  NLM     Status:  MEDLINE    
Matings between D. melanogaster females and males of sibling species in the D. melanogaster complex yield hybrid males that die prior to pupal differentiation. We have reexamined a previous report suggesting that the developmental defects in these lethal hybrid males reflect a failure in cell proliferation that may be the consequence of problems in mitotic chromosome condensation. We also observed a failure in cell proliferation, but find in contrast that the frequencies of mitotic figures and of nuclei staining for the mitotic marker phosphohistone H3 in the brains of hybrid male larvae are extremely low. We also found that very few of these brain cells in male hybrids are in S phase, as determined by BrdU incorporation. These data suggest that cells in hybrid males are arrested in either the G(1) or G(2) phases of the cell cycle. The cells in hybrid male brains appear to be particularly sensitive to environmental stress; our results indicate that certain in vitro incubation conditions induce widespread cellular necrosis in these brains, causing an abnormal nuclear morphology noted by previous investigators. We also document that hybrid larvae develop very slowly, particularly during the second larval instar. Finally, we found that the frequency of mitotic figures in hybrid male larvae mutant for Hybrid male rescue (Hmr) is increased relative to lethal hybrid males, although not to wild-type levels, and that chromosome morphology in Hmr(-) hybrid males is also not completely normal.
Bonnie J Bolkan; Ronald Booker; Michael L Goldberg; Daniel A Barbash
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-10-18
Journal Detail:
Title:  Genetics     Volume:  177     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-12     Completed Date:  2008-03-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2233-41     Citation Subset:  IM    
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853-2703, USA.
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MeSH Terms
Brain / pathology
Cell Cycle*
Chromosome Aberrations
Drosophila Proteins / genetics*
Drosophila melanogaster / cytology*,  growth & development*
Growth and Development
Larva / cytology
Grant Support
5R01GM048430-14/GM/NIGMS NIH HHS; 5R01GM074737-02/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Drosophila Proteins; 0/hybrid male rescue protein, Drosophila

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