| Developmental ability of trophoblast stem cells in uniparental mouse embryos. | |
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MedLine Citation:
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PMID: 19345411 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neither parthenogenetic (PG) nor androgenetic (AG) mouse embryos survive after day 9.5 of pregnancy, owing to the inadequate growth of extraembryonic tissues, including the placenta. At day 9.5 of pregnancy, the placental structures are poorly developed in PG embryos, while trophoblast giant cells are abundant at the implantation site in AG embryos. These findings suggest that both parental genomes are required for placental development. To gain further insight into the trophoblast lineage in PG and AG embryos, we attempted to derive trophoblast stem (TS)-like cell lines from uniparental embryos. Furthermore, we sought to assess their ability to differentiate into cells of the trophoblast lineage by using gene expression analysis. Three cell lines that expressed marker genes for undifferentiated TS cells (Cdx2 and Errbeta) were derived from AG embryos. Under differentiation conditions, these cells expressed the trophoblast giant cell-specific genes, but did not express the spongiotrophoblast-specific genes. In contrast, none of the four cell lines from PG embryos expressed marker genes for undifferentiated TS cells, but they expressed Oct3/4, a marker gene for embryonic stem cells. Immunohistochemical analysis indicated that PG blastocysts expressed Oct3/4 and Cdx2 specifically in inner cell mass and the trophectoderm respectively. These results suggest that PG embryos do not possess TS cells, because of the lack of the developmental ability of trophoblast cells. |
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Authors:
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H Ogawa; N Shindo; T Kumagai; Y Usami; M Shikanai; K Jonwn; A Fukuda; M Kawahara; Y Sotomaru; S Tanaka; T Arima; T Kono |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-03 |
Journal Detail:
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Title: Placenta Volume: 30 ISSN: 1532-3102 ISO Abbreviation: Placenta Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-28 Completed Date: 2009-09-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 448-56 Citation Subset: IM |
Affiliation:
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Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan. hogawa@nodai.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / physiology Cell Differentiation / genetics Cell Lineage / genetics Embryo, Mammalian / embryology* Embryonic Stem Cells / cytology, physiology* Female Fertilization in Vitro Gene Expression Regulation, Developmental Homeodomain Proteins / genetics Mice Octamer Transcription Factor-3 / genetics Parthenogenesis / genetics* Placenta / growth & development* Pregnancy Transcription Factors / genetics Trophoblasts / cytology* |
| Chemical | |
Reg. No./Substance:
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0/Cdx2 protein, mouse; 0/Homeodomain Proteins; 0/Octamer Transcription Factor-3; 0/Pou5f1 protein, mouse; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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