Document Detail

Developmental exposures of male rats to soy isoflavones impact Leydig cell differentiation.
MedLine Citation:
PMID:  20554919     Owner:  NLM     Status:  MEDLINE    
Testicular Leydig cells, which are the predominant source of the male sex steroid hormone testosterone, express estrogen receptors (ESRs) and are subject to regulation by estrogen. Following ingestion, the two major isoflavones in soybeans, genistin and daidzin, are hydrolyzed by gut microflora to form genistein and daidzein, which have the capacity to bind ESRs and affect gene expression. Thus, the increasing use of soy-based products as nondairy sources of protein has raised concerns about the potential of these products to cause reproductive toxicity. In the present study, perinatal exposure of male rats to isoflavones induced proliferative activity in Leydig cells. Isoflavones have the capacity to act directly as mitogens in Leydig cells, because genistein treatment induced Leydig cell division in vitro. Genistein action regulating Leydig cell division involved ESRs, acting in concert with signaling molecules in the transduction pathway mediated by protein kinase B (AKT) and mitogen-activated protein kinase (MAPK). Enhanced proliferative activity in the prepubertal period increased Leydig cell numbers, which alleviated deficits in androgen biosynthesis and/or augmented serum and testicular testosterone concentrations in adulthood. Together, these observations indicate that the perinatal exposures of male rats to isoflavones affected Leydig cell differentiation, and they imply that including soy products in the diets of neonates has potential implications for testis function.
Jessica D Sherrill; Morgan Sparks; John Dennis; Mahmoud Mansour; Barbara W Kemppainen; Frank F Bartol; Edward E Morrison; Benson T Akingbemi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-10
Journal Detail:
Title:  Biology of reproduction     Volume:  83     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  488-501     Citation Subset:  IM    
Department of Anatomy, Physiology, and Pharmacology, Auburn University, Auburn, Alabama 36849, USA.
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MeSH Terms
Analysis of Variance
Animals, Newborn
Blotting, Western
Cell Differentiation / drug effects*
Cell Proliferation / drug effects
Estradiol / biosynthesis
Isoflavones / pharmacology*
Leydig Cells / drug effects*,  metabolism
Mitogen-Activated Protein Kinases / metabolism
Rats, Long-Evans
Receptors, Androgen / metabolism
Receptors, LH / metabolism
Signal Transduction / drug effects
Testosterone / biosynthesis
Grant Support
Reg. No./Substance:
0/Isoflavones; 0/Receptors, Androgen; 0/Receptors, LH; 50-28-2/Estradiol; 58-22-0/Testosterone; EC Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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