Document Detail

Developmental Changes in the ECG of a Hamster Model of Muscular Dystrophy and Heart Failure.
Jump to Full Text
MedLine Citation:
PMID:  22629245     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Aberrant autonomic signaling is being increasingly recognized as an important symptom in neuromuscular disorders. The δ-sarcoglycan-deficient BIO TO-2 hamster is recognized as a good model for studying mechanistic pathways and sequelae in muscular dystrophy and heart failure, including autonomic nervous system (ANS) dysfunction. Recent studies using the TO-2 hamster model have provided promising preclinical results demonstrating the efficacy of gene therapy to treat skeletal muscle weakness and heart failure. Methods to accelerate preclinical testing of gene therapy and new drugs for neuromuscular diseases are urgently needed. The purpose of this investigation was to demonstrate a rapid non-invasive screen for characterizing the ANS imbalance in dystrophic TO-2 hamsters. Electrocardiograms were recorded non-invasively in conscious ∼9-month old TO-2 hamsters (n = 10) and non-myopathic F1B control hamsters (n = 10). Heart rate was higher in TO-2 hamsters than controls (453 ± 12 bpm vs. 311 ± 25 bpm, P < 0.01). Time domain heart rate variability, an index of parasympathetic tone, was lower in TO-2 hamsters (12.2 ± 3.7 bpm vs. 38.2 ± 6.8, P < 0.05), as was the coefficient of variance of the RR interval (2.8 ± 0.9% vs. 16.2 ± 3.4%, P < 0.05) compared to control hamsters. Power spectral analysis demonstrated reduced high frequency and low frequency contributions, indicating autonomic imbalance with increased sympathetic tone and decreased parasympathetic tone in dystrophic TO-2 hamsters. Similar observations in newborn hamsters indicate autonomic nervous dysfunction may occur quite early in life in neuromuscular diseases. Our findings of autonomic abnormalities in newborn hamsters with a mutation in the δ-sarcoglycan gene suggest approaches to correct modulation of the heart rate as prevention or therapy for muscular dystrophies.
Authors:
Thomas G Hampton; Ajit Kale; Scott McCue; Hemmi N Bhagavan; Case Vandongen
Related Documents :
10430765 - Diagnostic and prognostic value of serial dobutamine stress echocardiography for noninv...
15278415 - Dobutamine stress mri. part i. safety and feasibility of dobutamine cardiovascular magn...
12376005 - Clinical utility of low dose dobutamine echocardiography in regional myocardial viabili...
8074005 - Radionuclide studies in patients with stress-induced st-segment elevation after acute m...
25024745 - Hdac class i inhibitor, mocetinostat, reverses cardiac fibrosis in heart failure and di...
8698765 - Valve replacement with the carbomedics bileaflet mechanical prosthesis: clinical result...
Publication Detail:
Type:  Journal Article     Date:  2012-05-18
Journal Detail:
Title:  Frontiers in pharmacology     Volume:  3     ISSN:  1663-9812     ISO Abbreviation:  Front Pharmacol     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-25     Completed Date:  2012-10-02     Revised Date:  2013-08-13    
Medline Journal Info:
Nlm Unique ID:  101548923     Medline TA:  Front Pharmacol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  80     Citation Subset:  -    
Affiliation:
The Neuroscience Discovery Core Framingham, MA, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Front Pharmacol
Journal ID (iso-abbrev): Front Pharmacol
Journal ID (publisher-id): Front. Pharmacol.
ISSN: 1663-9812
Publisher: Frontiers Research Foundation
Article Information
Download PDF
Copyright © 2012 Hampton, Kale, McCue, Bhagavan and VanDongen.
open-access:
Received Day: 13 Month: 3 Year: 2012
Accepted Day: 12 Month: 4 Year: 2012
Electronic publication date: Day: 18 Month: 5 Year: 2012
collection publication date: Year: 2012
Volume: 3E-location ID: 80
ID: 3355504
PubMed Id: 22629245
DOI: 10.3389/fphar.2012.00080

Developmental Changes in the ECG of a Hamster Model of Muscular Dystrophy and Heart Failure
Thomas G. Hampton1*
Ajit Kale2
Scott McCue2
Hemmi N. Bhagavan3
Case VanDongen3
1The Neuroscience Discovery CoreFramingham, MA, USA
2The CuraVita Corporation, Mouse Specifics, Inc.Boston, MA, USA
3Research & Development, BioBreeders, Inc.Watertown, MA, USA
[edited-by] Edited by: Jufeng Wang, Waylandgreen, USA
[edited-by] Reviewed by: Suyash Prasad, BioMarin Pharmaceutical, USA; Domenico Criscuolo, Genovax, Italy
Correspondence: *Correspondence: Thomas G. Hampton, The Neuroscience Discovery Core, Worcester Road, Framingham, MA 01702, USA. e-mail: hampton@curavita.org
[other] This article was submitted to Frontiers in Pharmaceutical Medicine and Outcomes Research, a specialty of Frontiers in Pharmacology.

Introduction

Neuromuscular diseases, routinely considered with regard to muscle weakness, frequently affect the heart and the cardiovascular system. Aberrant autonomic signaling is being increasingly recognized as an important symptom in a variety of clinically defined neuromuscular disorders (Rakocevi’c-Stojanovi’c et al., 2007; Politano et al., 2008; Inoue et al., 2009; Della Marca et al., 2010). Patients with Duchenne muscular dystrophy, for example, often present with tachycardia and significant decreases in heart rate variability (HRV; Marcello et al., 1995; Yotsukura et al., 1998); cardiac failure is frequently the cause of death (Judge et al., 2011). There is a growing number of case reports presenting cardiac abnormalities in patients with spinal muscular atrophy (SMA; Hachiya et al., 2005; Rudnik-Schöneborn et al., 2008), a debilitating neurological disease marked predominantly by motor neuron death and atrophy of skeletal muscle. Cardiac muscle pathology sometimes exceeds skeletal muscle pathology in cases of limb-girdle muscular dystrophy (LGMD; Margeta et al., 2009). Many neuromuscular diseases affect children. Duchenne’s muscular dystrophy and some LGMD, childhood muscle disorders resulting from loss of expression of sarcoglycans, are both characterized by similar aberrant autonomic modulation of the heart in animal models (Giudice et al., 2000; Chu et al., 2002). There are some data that indicate that pathology that define the adult dystrophic phenotype originate in utero (Merrick et al., 2009). Here, we sought to determine if there is evidence of autonomic nervous system (ANS) abnormalities early in the life of δ-sarcoglycan-deficient hamsters, a model of LGMD characterized by definitive histopathological and clinical sequelae of skeletal muscle pathology and heart failure late in life (Straub et al., 1998; Xiao et al., 2000; Zhu et al., 2005; Kato et al., 2006; Hoshijima et al., 2011).


Materials and Methods
Animals

Male BIO F1B non-myopathic control and BIO TO-2 dystrophic hamsters were obtained from BioBreeders, Inc., Fitchburg, MA, USA. Handling and care of hamsters were consistent with federal guidelines and approved institutional protocols. Adults were studied at ∼9 months, an age with established clinical and histopathological evidence of skeletal and cardiac muscle disease (Zhu et al., 2005; Hoshijima et al., 2011). Newborn pups were studied at ∼4 and ∼11 days of age. The dams were removed from the nests prior to handling of the pups. A heat lamp was positioned near to a nest to provide ambient warmth to a pup in its mother’s absence. The time required to position a pup on the heated ECG recording platform (LifeSpoon™, Mouse Specifics, Inc., Boston) and acquire the ECG signal was ∼5 min, after which the pup was immediately returned to its dam.

ECG recordings

ECGs were recorded non-invasively in conscious hamsters similar to a method described previously for mice (Chu et al., 2001). Briefly, hamsters were gently removed from their cages and positioned inside the ECGenie™ recording enclosure (Mouse Specifics, Inc., Boston, MA, USA) sized to comfortably accommodate adult hamsters. A pair of silver-chloride ECG electrodes were embedded in the floor of the enclosure and spaced to provide contact between the electrodes and animals’ paws. Since even modest handling of hamsters may induce alterations in heart rate, each hamster was permitted to acclimatize for ∼10 min prior to collection of data. The signals were digitized at a sampling rate of 2000 samples/s. When hamsters were positioned such that a forepaw and hind paw were not uniquely in contact with one of the electrodes, the output from the amplifier was discarded. Only data from continuous recordings were used in the analyses. Each signal was analyzed using e-MOUSE™, which incorporates Fourier analyses and linear time-invariant digital filtering of frequencies below 3 Hz and above 100 Hz to minimize environmental signal disturbances. The software uses a peak detection algorithm to find the peak of the R-waves and to calculate heart rate. Subsequently, determination of first and second derivatives and algebraic “if-then” search the ECG signals for probable P-wave peaks and onset and termination of QRS complexes. The end of the T-wave of each signal was defined as the point where the signal returned to the isoelectric line (the mean voltage between the preceding P-wave and QRS interval). The QT intervals were rate corrected (QTc) by application of an equation recommended for rodent electrocardiography (Mitchell et al., 1998). The software plots its interpretation of P, Q, R, S, and T for each beat so that spurious data resulting from unfiltered noise or motion artifacts may be rejected. e-MOUSE™ then calculates the mean of the ECG time intervals for each set of waveforms. Figure 1A depicts a F1B control hamster exploring an ECGenie™ recording enclosure.

For electrocardiographic monitoring of the neonates, hamster pups were positioned atop of the LifeSpoon™ ECG recording module, the surface of which is embedded with two heated silver-chloride electrodes to maintain a warmed environment similar to that provided by the nursing mother and nest. Surprisingly, even at ∼4 days of age, the hamster pups were initially quite ambulatory, but became quiescent after ∼5 min at which time ∼20–30 complexes were recorded.

Measures of heart beat-to-beat variability (HRV) were determined as the SD and the coefficient of variation (CV). The SD reflects the dispersion about the average value for a parameter. CV, expressed as a%, was calculated from the following equation: 100 × SD/mean value. Power spectral analysis of ECG in the frequency domain for analysis of HRV was accomplished using low frequency (LF) and high frequency (HF) ranges similar to those previously published for hamsters (Giudice et al., 2000). The LF power range was set from 0.04 to 1.0 Hz, and the HF power range was set from 1.0 to 3.0 Hz.

Statistics

Data are presented as the mean ± SE. Group means comparisons between strains, or among strains between ages, were performed using Student’s two-tailed t-test for unpaired observations. Differences were considered significant with P < 0.05.


Results

We recorded ECGs non-invasively in 10 conscious F1B control hamsters and 10 TO-2 δ-sarcoglycan-deficient hamsters, an animal model used routinely to test the efficacy of new therapies in treating muscular dystrophies and heart failure. A representative ECG recording from a ∼9-month old TO-2 hamster is shown in Figure 1B, a subset of ∼100 continuous ECG complexes acquired over ∼15 s. Since the hamsters are conscious, baseline artifact and noise are apparent in the unfiltered signals. Yet, the P-waves and T-waves are discernible by eye and interpretable by the software algorithmic processing of the signal digitized at 2000 samples/s. Any discontinuities in the signal resulting from loss of contact between the paws and the electrodes were excluded from analysis. The hamster ECG is more typical of that of mouse and rat in that there is no separate T-wave (Bestetti and Oliveira, 1990; Chu et al., 2001), and unlike in guinea pigs where there is a separate T-wave (Gussak et al., 2000).

Analyses of the digitized ECG signals demonstrated significantly higher heart rate in TO-2 dystrophic hamsters than in F1B controls (453 ± 12 bpm vs. 311 ± 25 bpm, P < 0.01). Table 1 summarizes the ECG observations, which include shorter PR duration and QT interval in TO-2 than in F1B hamsters. Rate corrected QT (QTc) in TO-2 hamsters was significantly shorter than in F1B hamsters (59.3 ± 0.9 ms vs. 69.5 ± 2.5 ms, P < 0.05).

Heart rate variability was significantly lower in TO-2 hamsters compared to F1B control hamsters as reflected by HRV (12.2 ± 3.7 bpm vs. 38.2 ± 6.8 bpm, P < 0.05) and CV (2.8 ± 0.9% vs. 16.2 ± 3.4%, P < 0.05; Table 1). Figure 2 illustrates RR tachograms, or analysis of variations in the instantaneous heart rate time series using the beat-to-beat RR intervals, to reflect HRV. Note the lower beat-to-beat variability in the RR intervals in the cardiomyopathic TO-2 hamster, reflecting the lower HRV in these animals.

Analysis of HRV in the frequency domain further characterized reduced HRV in TO-2 hamsters. Fourier transformation of the beat-to-beat interval duration series yielded power spectral densities, from which total power, LF, and HF values were computed. Total power was reduced in cardiomyopathic TO-2 hamsters compared to controls (3.0 ± 1.2 ms2 vs. 881.6 ± 293.8 ms2, P < 0.05). The LF component, reflecting mainly sympathetic influences, was lower in TO-2 than in controls (1.2 ± 0.6 ms2 vs. 502.8 ± 165.3 ms2, P < 0.05). The HF spectral component, reflecting mainly parasympathetic influences, was also lower in cardiomyopathic TO-2 than in F1B hamsters (1.6 ± 0.6 ms2 vs. 335.2 ± 125.7 ms2, P < 0.05). Figure 3 illustrates the significantly lower HRV revealed by power spectral analysis in TO-2 dystrophic hamsters.

Reducing the spacing of the electrodes and providing heat to the ECG recording surface enabled recording of the ECG in newborn F1B hamsters (n = 3) and TO-2 hamsters (n = 4) and return them to their dams. Figure 4A depicts a hamster pup inside the LifeSpoon™ ECG recording chamber; Figure 4B shows representative complexes from a ∼4-day old TO-2 hamster pup. The heart rate in the neonates, 208 ± 11 bpm in F1B pups and 248 ± 15 bpm in TO-2 pups, was significantly lower than heart rate in the adult hamsters (P < 0.05), consistent with the relative bradycardia that has been reported for neonatal mice (Heier et al., 2010) and rats (Hofer and Reiser, 1969). Heart rate was significantly higher, however, in ∼4-day old TO-2 hamsters than F1B control hamsters (P < 0.05). HRV in F1B neonates was significantly lower than in adult F1B hamsters (15.7 ± 8.3 bpm vs. 38.2 ± 6.8, P < 0.05). HRV in developing TO-2 hamsters, moreover, was not different from adult TO-2 hamsters.

Autonomic differences were also apparent by ∼11 days of age. HRV was significantly lower in TO-2 hamsters (n = 5) than in F1B hamsters (n = 5; 8.8 ± 0.9 bpm vs. 16.3 ± 3.1 bpm, P < 0.05) as was CV (5.1 ± 0.6% vs. 9.9 ± 0.2%, P < 0.05). Total power and the LF component were significantly lower in ∼11-day old TO-2 hamsters than in F1B controls. The ratio of the LF to HF component, a strong indicator of sympathetic tone (Mongue-Din et al., 2009), was also significantly lower in ∼11-day old δ-sarcoglycan-deficient TO-2 hamsters. These data indicate that autonomic dysfunction begins early in life in these overtly healthy animals that go on to develop pathological evidence and clinical signs of muscular dystrophy and heart failure.


Discussion

This report describes a simple and elegant system for recording ECGs in conscious newborn and adult hamsters; neither attachment of wires, nor anesthesia, nor surgical implantation of devices is required. The ECG measurements we obtained non-invasively in conscious adult hamsters are comparable to those obtained using subcutaneously implanted electrodes (Giudice et al., 2000) and implantable telemetry devices (Mongue-Din et al., 2009; Wang et al., 2011). The data we obtained non-invasively in awake neonatal δ-sarcoglycan-deficient TO-2 hamsters suggest that the ANS abnormalities clearly apparent in older moribund myopathic TO-2 animals may actually commence very early in life.

The δ-sarcoglycan-deficient BIO TO-2 hamster is an established model of human LGMD-type 2F muscular dystrophy and heart failure. Whereas muscle weakness, predominantly affecting the pelvic and shoulder girdles, is the characteristic feature in patients with LGMD, cardiomyopathy is frequently the cause of death. The TO-2 hamster model recapitulates these characteristics. Pathology has indicated damage to the intercostal muscles, proximal limb muscles, muscles of the pelvic girdle, shoulder, gastrocnemius, triceps, and tibialis muscles (Straub et al., 1998; Xiao et al., 2000; Zhu et al., 2005). Skeletal muscle weakness and pathology are already evident in TO-2 animals at ∼1 month of age (Homburger, 1979; Straub et al., 1998; Hampton et al., 2011). These hamsters, however, also develop cardiac pathology apparent after ∼3 months of age. Cardiac fibrosis and calcification lead to dilation; congestive heart failure is usually the cause of death in TO-2 hamsters (Xiao et al., 2000; Zhu et al., 2005; Kato et al., 2006; Hoshijima et al., 2011).

Healthy modulation of the heart rhythm is influenced by the ANS, with contributions from the parasympathetic and sympathetic limbs of the ANS enabling a fair amount of plasticity in heart rate. Reduced HRV is an established marker of autonomic dysfunction and is associated with increased mortality in patients with heart failure. To the best of our knowledge, there are two descriptions of reduced HRV and autonomic dysfunction in TO-2 hamsters. Researchers (Giudice et al., 2000) described a procedure of surgical implantation of radio transmitters followed by a recovery period to report heart rate and HRV data quite similar to the values we obtained non-invasively. They demonstrated autonomic imbalance of cardiac control due to attenuation of parasympathetic activity and enhanced sympathetic tone in TO-2 hamsters in heart failure. Our observations of reductions in the total spectral power, the LF component, and the HF component, are in agreement with those they reported. A subsequent study in cardiomyopathic TO-2 hamsters, also reporting similar values for heart rate and HRV as we report here, demonstrated that the lusitropic agent istaroxime improved cardiac function via preservation of autonomic tone (Lo Giudice et al., 2011). Changes in autonomic modulation of the heart in TO-2 cardiomyopathic hamsters was thought to be a reactive compensation to impaired cardiac function (Giudice et al., 2000). Our novel observations of autonomic dysregulation of heart rhythm in neonatal TO-2 hamsters, however, suggests that autonomic dysfunction might be a causative factor in the etiology of skeletal and cardiac muscle disease.

Autonomic nervous system dysfunction of the heart in TO-2 hamsters can be quickly and non-invasively quantified. To what extent unabated sympathetic overdrive commencing early in life contributes to the eventual demise of skeletal and cardiac muscle remains to be determined.

The method we describe for recording ECG in newborn rodents and our observations of neonatal autonomic disturbances are of practical significance in accelerating the testing of new therapeutic modalities in the treatment of muscular dystrophies and heart failure.


Conflict of Interest Statement

T. G. Hampton is owner of Mouse Specifics, Inc., a company that has commercialized the non-invasive ECG instrumentation described in Materials and Methods. C. G. VanDongen is owner of BioBreeders, Inc., a purveyor of hamsters.


Cecelia Hampton is acknowledged for her helpful comments on the manuscript.


References
Bestetti R. B.,Oliveira J. S.. (Year: 1990). The surface electrocardiogram: a simple and reliable method for detecting overt and latent heart disease in rats. Braz. J. Med. Biol. Res.23, 1213–12222136553
Chu V.,Otero J. M.,Lopez O.,Morgan J. P.,Amende I.,Hampton T. G.. (Year: 2001). Method for non-invasively recording electrocardiograms in conscious mice. BMC Physiol.1, 610.1186/1472-6793-1-611476671
Chu V.,Otero J. M.,Lopez O.,Sullivan M. F.,Morgan J. P.,Amende I.,Hampton T. G.. (Year: 2002). Electrocardiographic findings in mdx mice: a cardiac phenotype of Duchenne muscular dystrophy. Muscle Nerve26, 513–51910.1002/mus.1022312362417
Della Marca G.,Frusciante R.,Scatena M.,Testani E.,Vollono C.,Losurdo A.,Scarano E.,Colicchio S.,Farina B.,Gnoni V.,Mazza S.,Tonali P. A.,Ricci E.. (Year: 2010). Heart rate variability in facioscapulohumeral muscular dystrophy. Funct. Neurol.25, 211–21621388582
Giudice P. L.,Gagnol J. P.,Bellucci A.,Buffone G.,Careddu A.,Magni G.,Quagliata T.,Pacifici L.,Carminati P.. (Year: 2000). Autonomic nervous system activity imbalance in cardiomyopathic hamster. J. Cardiovasc. Pharmacol.36, 369–37510.1097/00005344-200009000-0001310975595
Gussak I.,Chaitman B. R.,Kopecky S. L.,Nerbonne J. M.. (Year: 2000). Rapid ventricular repolarization in rodents: electrocardiographic manifestations, molecular mechanisms, and clinical insights. J. Electrocardiol.33, 159–17010.1054/jelc.2000.1810610819409
Hachiya Y.,Arai H.,Hayashi M.,Kumada S.,Furushima W.,Ohtsuka E.,Ito Y.,Uchiyama A.,Kurata K.. (Year: 2005). Autonomic dysfunction in cases of spinal muscular atrophy type 1 with long survival. Brain Dev.27, 574–57810.1016/j.braindev.2005.02.00915876504
Hampton T. G.,Kale A.,Amende I.,Tang W.,McCue S.,Bhagavan H. N.,VanDongen C. G.. (Year: 2011). Gait disturbances in dystrophic hamsters. J. Biomed. Biotechnol.201110.1155/2011/23535421318074
Heier C. R.,Hampton T. G.,Wang D.,Didonato C. J.. (Year: 2010). Development of electrocardiogram intervals during growth of FVB/N neonate mice. BMC Physiol.10, 1610.1186/1472-6793-10-1620735846
Hofer M. A.,Reiser M. F.. (Year: 1969). The development of cardiac rate regulation in preweanling rats. Psychosom. Med.31, 372–3885350296
Homburger F.. (Year: 1979). Myopathy of hamster dystrophy: history and morphologic aspects. Ann. N. Y. Acad. Sci.317, 1–1710.1111/j.1749-6632.1979.tb56503.x382952
Hoshijima M.,Hayashi T.,Jeon Y. E.,Fu Z.,Gu Y.,Dalton N. D.,Ellisman M. H.,Xiao X.,Powell F. L.,Ross J. Jr.. (Year: 2011). Delta-sarcoglycan gene therapy halts progression of cardiac dysfunction, improves respiratory failure, and prolongs life in myopathic hamsters. Circ. Heart Fail.4, 89–9710.1161/CIRCHEARTFAILURE.110.95725821036890
Inoue M.,Mori K.,Hayabuchi Y.,Tatara K.,Kagami S.. (Year: 2009). Autonomic function in patients with Duchenne muscular dystrophy. Pediatr. Int.51, 33–4010.1111/j.1442-200X.2008.02656.x19371275
Judge D. P.,Kass D. A.,Thompson W. R.,Wagner K. R.. (Year: 2011). Pathophysiology and therapy of cardiac dysfunction in Duchenne muscular dystrophy. Am. J. Cardiovasc. Drugs11, 287–29410.2165/11594070-000000000-0000021812510
Kato Y.,Iwase M.,Takagi K.,Nishizawa T.,Kanazawa H.,Matsushita A.,Umeda H.,Izawa H.,Noda A.,Koike Y.,Nagata K.,Yokota M.. (Year: 2006). Differential myolysis of myocardium and skeletal muscle in hamsters with dilated cardiomyopathy – beneficial protective effect of diltiazem. Circ. J.70, 1497–150210.1253/circj.70.165817062977
Lo Giudice P.,Mattera G. G.,Gagnol J. P.,Borsini F.. (Year: 2011). Chronic istaroxime improves cardiac function and heart rate variability in cardiomyopathic hamsters. Cardiovasc. Drugs Ther.25, 133–13810.1007/s10557-011-6283-y21287411
Marcello N.,Baratti M.,Ortaggio F.,Vescovini E.,Zanoni P.,Tugnoli V.,De Grandis D.. (Year: 1995). Sympathetic skin response in patients with Duchenne muscular dystrophy. Electromyogr. Clin. Neurophysiol.35, 387–3958549429
Margeta M.,Connolly A. M.,Winder T. L.,Pestronk A.,Moore S. A.. (Year: 2009). Cardiac pathology exceeds skeletal muscle pathology in two cases of limb-girdle muscular dystrophy type 2I. Muscle Nerve40, 883–88910.1002/mus.2143219705481
Merrick D.,Stadler L. K.,Larner D.,Smith J.. (Year: 2009). Muscular dystrophy begins early in embryonic development deriving from stem cell loss and disrupted skeletal muscle formation. Dis. Model. Mech.2, 374–38810.1242/dmm.00100819535499
Mitchell G. F.,Jeron A.,Koren G.. (Year: 1998). Measurement of heart rate and Q-T interval in the conscious mouse. Am. J. Physiol.274(Pt 2), H747–H7519530184
Mongue-Din H.,Salmon A.,Fiszman M. Y.,Fromes Y.. (Year: 2009). Periodic variation in R-R intervals and cardiovascular autonomic regulation in young adult Syrian hamsters. Am. J. Physiol. Regul. Integr. Comp. Physiol.296, R610–R61710.1152/ajpregu.90376.200819109374
Politano L.,Palladino A.,Nigro G.,Scutifero M.,Cozza V.. (Year: 2008). Usefulness of heart rate variability as a predictor of sudden cardiac death in muscular dystrophies. Acta Myol.27, 114–12219472920
Rakocevi’c-Stojanovi’c V.,Milovanovi’c B.,Ivi’c N.,Ille T.,Marjanovi’c I.,Stevi’c Z.,Pavlovi’c S.,Lavrni’c D.. (Year: 2007). Cardiac autonomic nervous system in patients with myotonic dystrophy type 1. Acta Myol.26, 112–11418421899
Rudnik-Schöneborn S.,Heller R.,Berg C.,Betzler C.,Grimm T.,Eggermann T.,Eggermann K.,Wirth R.,Wirth B.,Zerres K.. (Year: 2008). Congenital heart disease is a feature of severe infantile spinal muscular atrophy. J. Med. Genet.45, 635–63810.1136/jmg.2008.05795018662980
Straub V.,Duclos F.,Venzke D. P.,Lee J. C.,Cutshall S.,Leveille C. J.,Campbell K. P.. (Year: 1998). Molecular pathogenesis of muscle degeneration in the δ-sarcoglycan-deficient hamster. Am. J. Pathol.153, 1623–163010.1016/S0002-9440(10)65751-39811355
Wang H.,Siddharthan V.,Hall J. O.,Morrey J. D.. (Year: 2011). Autonomic nervous dysfunction in hamsters infected with West Nile virus. PLoS ONE6, e1957510.1371/journal.pone.001957521573009
Xiao X.,Li J.,Tsao Y. P.,Dressman D.,Hoffman E. P.,Watchko J. F.. (Year: 2000). Full functional rescue of a complete muscle (TA) in dystrophic hamsters by adeno-associated virus vector-directed gene therapy. J. Virol.74, 1436–144210.1128/JVI.74.3.1436-1442.200010627554
Yotsukura M.,Fujii K.,Katayama A.,Tomono Y.,Ando H.,Sakata K.,Ishihara T.,Ishikawa K.. (Year: 1998). Nine-year follow-up study of heart rate variability in patients with Duchenne-type progressive muscular dystrophy. Am. Heart J.136, 289–29610.1053/hj.1998.v136.897379704692
Zhu T.,Zhou L.,Mori S.,Wang Z.,McTiernan C. F.,Qiao C.,Chen C.,Wang D. W.,Li J.,Xiao X.. (Year: 2005). Sustained whole-body functional rescue in congestive heart failure and muscular dystrophy hamsters by systemic gene transfer. Circulation112, 2650–265910.1161/CIRCULATIONAHA.104.51902516230483

Figures

[Figure ID: F1]
Figure 1 

(A) Adult (9 months old) F1B control hamster exploring an ECGenie™ recording enclosure just prior to the lid being closed to prevent escape; to the right a TO-2 hamster can be seen inside an ECG recording enclosure. The ECG is detected passively through the underside of the subject’s paws as it rests atop two electrodes embedded in the enclosure floor. (B) Representative ECG signals, a subset from ∼100 continuous ECG complexes recorded over ∼15 s, acquired non-invasively from a conscious adult TO-2 hamster. The PQRST morphology is indicated; note the high regularity in the RR intervals in this cardiomyopathic animal.



[Figure ID: F2]
Figure 2 

RR tachograms, or analysis of variations in the instantaneous heart rate time series using the beat-to-beat RR intervals, to reflect heart rate variability. Beat-to-beat variability in the RR interval durations was very low in cardiomyopathic TO-2 hamsters, reflecting altered autonomic heart rate modulation in comparison to F1B control hamsters.



[Figure ID: F3]
Figure 3 

Power spectral analysis indicated lower total power, comprised of reduced LF (sympathetic influences) and reduced HF (parasympathetic influences) components. These data further characterize the abnormal autonomic balance in TO-2 dystrophic hamsters.



[Figure ID: F4]
Figure 4 

(A) Neonate TO-2 hamster (4 days old) resting on warmed LifeSpoon ECG recording platform. (B) ECG tracings from neonatal (∼4 days old) F1B control hamster. Heart rate was significantly higher, and heart rate variability significantly lower, in TO-2 dystrophic hamsters, indicative of autonomic dysfunction, beginning very early in life.



Tables
[TableWrap ID: T1] Table 1 

Electrocardiographic parameters in BIO F1B and BIO TO-2 conscious hamsters.


F1B (n = 10) TO-2 (n = 10)
Heart rate (bpm) 311 ± 25 453 ± 12*
HR variability (bpm) 38.2 ± 6.8 12.2 ± 3.7*
CV (%) 16.2 ± 3.4 2.8 ± 0.9*
PR (ms) 53.8 ± 4.2 36.8 ± 2.1*
QRS (ms) 23.5 ± 1.7 19.1 ± 1.2
QT (ms) 99.3 ± 6.2 68.5 ± 1.9*
QTc (ms) 69.5 ± 2.5 59.3 ± 0.9*

Values are mean ± SE.

HR var, heart rate variability; CV, coefficient of variation; QTc, QT corrected for heart rate.

tfn1*P < 0.05 vs. F1B hamsters.



Article Categories:
  • Pharmacology
    • Review Article

Keywords: muscular dystrophy, autonomic nervous system, heart failure, development, cardiomyopathy, hamsters, δ-sarcoglycan deficiency, BIO TO-2 hamsters.

Previous Document:  The budget impact of oral nutritional supplements for disease related malnutrition in elderly in the...
Next Document:  Transport and metabolism at blood-brain interfaces and in neural cells: relevance to bilirubin-induc...