Document Detail

Developmental acquisition of the Lyn-CD22-SHP-1 inhibitory pathway promotes B cell tolerance.
MedLine Citation:
PMID:  19380785     Owner:  NLM     Status:  MEDLINE    
To better understand whether autoimmunity in Lyn-deficient mice arises from compromised central or peripheral B cell tolerance, we examined BCR signaling properties of wild-type and Lyn-deficient B cells at different stages of development. Wild-type mature follicular B cells were less sensitive to BCR stimulation than were immature transitional stage 1 B cells with regard to BCR-induced calcium elevation and ERK MAPK activation. In the absence of Lyn, mature B cell signaling was greatly enhanced, whereas immature B cell signaling was minimally affected. Correspondingly, Lyn deficiency substantially enhanced the sensitivity of mature B cells to activation via the BCR, but minimally affected events associated with tolerance induction at the immature stage. The effects of CD22 deficiency on BCR signaling were very similar in B cells at different stages of maturation. These results indicate that the Lyn-CD22-Src homology region 2 domain-containing phosphatase-1 inhibitory pathway largely becomes operational as B cell mature, and sets a threshold for activation that appears to be critical for the maintenance of tolerance in the B cell compartment.
Andrew J Gross; Julia R Lyandres; Anil K Panigrahi; Eline T Luning Prak; Anthony L DeFranco
Related Documents :
8632795 - Two distinct mechanisms for long-range patterning by decapentaplegic in the drosophila ...
10887085 - Novel cell-cell interactions during vulva development in pristionchus pacificus.
16678095 - Wntless, a conserved membrane protein dedicated to the secretion of wnt proteins from s...
18762575 - Ranbpm regulates cell shape, arrangement, and capacity of the female germline stem cell...
16956395 - Optimized detection of circulating anti-nuclear envelope autoantibodies by immunofluore...
15616745 - Design and fabrication of an integrated cell processor for single embryo cell manipulat...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  182     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-21     Completed Date:  2009-06-15     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5382-92     Citation Subset:  AIM; IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
B-Lymphocyte Subsets / cytology,  enzymology,  immunology*,  metabolism
Cell Differentiation / genetics,  immunology
Gene Expression Regulation, Developmental / physiology*
Immune Tolerance* / genetics
Mice, Inbred C57BL
Mice, Knockout
Mice, Mutant Strains
Mice, Transgenic
Protein Tyrosine Phosphatase, Non-Receptor Type 6 / deficiency,  genetics*,  physiology
Receptors, Antigen, B-Cell / antagonists & inhibitors*,  genetics,  physiology
Sialic Acid Binding Ig-like Lectin 2 / genetics*,  metabolism,  physiology
Signal Transduction / genetics,  immunology*
Spleen / cytology,  enzymology,  immunology
Up-Regulation / genetics,  immunology
src-Family Kinases / deficiency,  genetics*,  physiology
Grant Support
Reg. No./Substance:
0/Cd22 protein, mouse; 0/Receptors, Antigen, B-Cell; 0/Sialic Acid Binding Ig-like Lectin 2; EC protein-tyrosine kinase; EC Kinases; EC Tyrosine Phosphatase, Non-Receptor Type 6; EC protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  ATLa, an Aspirin-Triggered Lipoxin A4 Synthetic Analog, Prevents the Inflammatory and Fibrotic Effec...
Next Document:  Ym1/2 promotes th2 cytokine expression by inhibiting 12/15(s)-lipoxygenase: identification of a nove...