| Development of zona-free hamster ova to blastocysts in vitro. | |
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MedLine Citation:
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PMID: 11097037 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The zona pellucida (ZP) enclosing the mammalian ovum is important for its protection and for initial stages of fertilization, but the role of the ZP during embryo development is less clear. This study was designed to investigate if the hamster ZP is needed for embryo development from 1-cell to blastocyst in vitro, and to compare methods for removing the ZP. A total of 395 hamster pronucleate ova were collected 10 h post activation from superovulated, mated female hamsters. The ZP was removed from some ova using either 0.05% pronase, 0.05% trypsin or acid Tyrode's solution. To prevent ZP-free ova from sticking together, they were cultured singly in 30-50 microL drops of HECM-6 culture medium together with ZP-intact ova as controls. There was no significant difference among treatment groups in embryo development to blastocyst: 36/87 (42%) in the ZP intact group; 35/75 (47%) in the pronase-treated ZP-free group; 37/74 (50%) in the trypsin-treated ZP-free group; and 37/71 (52%) in the acid-treated ZP-free group. These results indicate that 1) the ZP is unnecessary for hamster embryo development in vitro from the pronucleate ovum stage to blastocyst; 2) none of the three ZP-removal methods was detrimental to embryo development; 3) embryos do not need to be cultured in groups during in vitro development from 1-cell to blastocyst. |
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Authors:
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W Ji; B D Bavister |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Theriogenology Volume: 54 ISSN: 0093-691X ISO Abbreviation: Theriogenology Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2001-01-08 Completed Date: 2001-01-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 827-34 Citation Subset: IM |
Affiliation:
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Kunming Institute of Zoology, the Chinese Academy of Sciences, China. wji@mail.kiz.ac.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzimidazoles / chemistry Blastocyst / chemistry, physiology* Cell Culture Techniques Chorionic Gonadotropin / administration & dosage Cricetinae Female Fluorescent Dyes / chemistry Isotonic Solutions / chemistry Male Mesocricetus / embryology* Microscopy, Fluorescence / veterinary Ovum / growth & development, physiology* Pronase / chemistry Trypan Blue / chemistry Trypsin / chemistry Zona Pellucida / chemistry, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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HD22023/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Benzimidazoles; 0/Chorionic Gonadotropin; 0/Fluorescent Dyes; 0/Isotonic Solutions; 0/Tyrode's solution; 23491-52-3/HOE 33342; 72-57-1/Trypan Blue; EC 3.4.21.4/Trypsin; EC 3.4.24.-/Pronase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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