Document Detail

Development of a voltammetric technique for monitoring brain dopamine metabolism: compensation for interference caused by DOPAC electrogenerated during homovanillic acid detection.
MedLine Citation:
PMID:  19381381     Owner:  NLM     Status:  In-Process    
The established stability of carbon-paste electrodes (CPEs) in brain extracellular fluid was exploited to develop a voltammetric technique to monitor the dopamine metabolite, homovanillic acid (HVA), at 10 s intervals. At the scan rates needed for this time resolution, 3,4-dihydroxyphenylacetic acid (DOPAC), electrogenerated as a result of HVA oxidation, was observed in the cyclic staircase voltammograms, and this interfered with the straightforward reliable quantification of HVA. However, correction of the HVA signal, recorded in mixtures, with currents from the DOPAC and ascorbate regions of the voltammogram allowed the reproducible construction of well behaved HVA calibration plots. These showed good linearity, LOD values, selectivity and stability during six days of continuous CPE exposure to a lipid medium, which served as an in-vitro model of CPE implantation in brain tissue for future applications.
Ismael A L Mulla; John P Lowry; Pier Andrea Serra; Robert D O'Neill
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-05
Journal Detail:
Title:  The Analyst     Volume:  134     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  893-8     Citation Subset:  IM    
UCD School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
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