Document Detail

Development and validation of a simple HPLC method for simultaneous in vitro determination of amoxicillin and metronidazole at single wavelength.
MedLine Citation:
PMID:  16859857     Owner:  NLM     Status:  MEDLINE    
A simple, rapid, sensitive and robust reversed phase-HPLC method was developed and validated to measure simultaneously the amount of amoxicillin and metronidazole at single wavelength (254 nm) in order to assess drug release profiles and drug-excipients compatibility studies for a new floating-sustained release tablet formulation and its subsequent stability studies. An isocratic elution of filtered sample was performed on C18 column with buffered mobile phase (pH 4.0) and UV detection at 254 nm. Quantification was achieved with reference to the external standards. The linearity for concentrations between 0.15 and 600 microg/ml for amoxicillin and 0.13 and 300 microg/ml for metronidazole were established. Intra and inter-day precision were less than 2.5%. The limits of detection (LOD) and quantification were 0.05 and 0.15 microg/ml for amoxicillin and 0.10 and 0.13 microg/ml for metronidazole. The determination of the two active ingredients was not interfered by the excipients of the products. Samples were stable in the release media (37 degrees C) and the HPLC injector at least for 12 h.
Naser Tavakoli; Jaleh Varshosaz; Farid Dorkoosh; Mohammad R Zargarzadeh
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Publication Detail:
Type:  Journal Article     Date:  2006-07-21
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  43     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-12     Completed Date:  2007-03-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  325-9     Citation Subset:  IM    
Faculty of Pharmacy & Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
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MeSH Terms
Amoxicillin / analysis*
Chemistry, Pharmaceutical
Chromatography, High Pressure Liquid
Delayed-Action Preparations
Drug Stability
Indicators and Reagents
Metronidazole / analysis*
Penicillins / analysis*
Quality Control
Reference Standards
Reproducibility of Results
Spectrophotometry, Ultraviolet
Reg. No./Substance:
0/Delayed-Action Preparations; 0/Indicators and Reagents; 0/Penicillins; 0/Tablets; 26787-78-0/Amoxicillin; 443-48-1/Metronidazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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