Document Detail

Development and validation of sensitive liquid chromatography/tandem mass spectrometry method for quantification of bendamustine in mouse brain tissue.
MedLine Citation:
PMID:  22925718     Owner:  NLM     Status:  MEDLINE    
A liquid chromatography-tandem mass spectrometry method for quantification of bendamustine in mouse brain tissue was developed and fully validated. Methanol was used to precipitate proteins in brain tissue. Bendamustine and internal standard (chlorambucil) were separated with reverse-phase chromatography on a C-18 column with a gradient of water and 95% methanol in 0.1% formic acid. Positive mode electrospray ionization was applied with selected reaction monitoring to achieve 5 ng/ml lower limits of quantitation in mouse brain tissue. The calibration curve for bendamustine in mouse brain was linear between 5 and 2000 ng/ml. The within- and between-batch accuracy and precision of the assay were within 15% at 10, 100 and 1000 ng/ml. The recovery and matrix effect of bendamustine in mouse brain tissue ranged from 41.1% to 51.6% and 107.4% to 110.3%, respectively. The validated method was then applied to quantitate bendamustine in an animal study. Results indicate the assay can be applied to evaluate bendamustine disposition in mouse brain tissue. This assay will be applied in the future to detect and quantify bendamustine in human brain tissue samples.
Lei He; John C Grecula; Yonghua Ling; Michael D Enzerra; Mario Ammirati; Kari Kendra; Robert Cavaliere; Nina Mayr; John McGregor; Thomas Olencki; Ewa Mrozek; Mani Matharbootham; Chima Oluigbo; Mitch A Phelps
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-16
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  905     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-10     Completed Date:  2013-02-28     Revised Date:  2013-12-17    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  141-4     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
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MeSH Terms
Brain Chemistry*
Chromatography, Liquid / methods*
Drug Stability
Least-Squares Analysis
Mice, Inbred ICR
Nitrogen Mustard Compounds / analysis*
Reproducibility of Results
Sensitivity and Specificity
Tandem Mass Spectrometry / methods*
Grant Support
Reg. No./Substance:
0/Nitrogen Mustard Compounds; 9266D9P3PQ/bendamustine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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