Document Detail

Development and validation of a sandwich ELISA for the determination of potentially allergenic sesame (Sesamum indicum) in food.
MedLine Citation:
PMID:  20700583     Owner:  NLM     Status:  MEDLINE    
This paper presents a sandwich enzyme-linked immunosorbent assay (ELISA) that allows the determination of traces of sesame in food. Chicken anti-sesame antibodies, used as coating antibodies, and rabbit anti-sesame antibodies, used as secondary antibodies, were prepared by immunization with a protein extract of white, peeled sesame. The ELISA did not show any cross-reactivity with 19 food ingredients commonly found in sesame-containing foodstuffs such as seeds, nuts, and cereals. In whole grain bread, crisp toast, and snacks, the limit of detection (S/N = 3) was 0.5, 0.5, and 0.3 μg sesame protein/g, and the limit of quantification (S/N = 10) was 0.6, 0.8, and 1.4 μg sesame protein/g, respectively. The analysis of blank food matrices (whole grain bread, white bread, crisp toast, and snacks) spiked with sesame protein at four spike levels generally resulted in mean recoveries from 72% to 145%. In the case of spiking blank food matrices with sesame seeds, the ELISA proved to be more accurate for whole wheat cookies than for whole wheat bread.
Gerda Redl; Fatima T Husain; Ines E Bretbacher; Albert Nemes; Margit Cichna-Markl
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Validation Studies     Date:  2010-08-11
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  398     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-22     Completed Date:  2011-01-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1735-45     Citation Subset:  IM    
Department of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria.
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MeSH Terms
Allergens / analysis*,  immunology
Enzyme-Linked Immunosorbent Assay / methods*
Plant Proteins / analysis*,  immunology
Seeds / chemistry,  immunology
Sesamum / chemistry*,  immunology
Reg. No./Substance:
0/Allergens; 0/Plant Proteins

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