| Development and validation of a rapid multi-class method for the confirmation of fourteen prohibited medicinal additives in pig and poultry compound feed by liquid chromatography-tandem mass spectrometry. | |
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MedLine Citation:
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PMID: 20643524 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A confirmatory method has been developed to allow for the analysis of fourteen prohibited medicinal additives in pig and poultry compound feed. These compounds are prohibited for use as feed additives although some are still authorised for use in medicated feed. Feed samples are extracted by acetonitrile with addition of sodium sulfate. The extracts undergo a hexane wash to aid with sample purification. The extracts are then evaporated to dryness and reconstituted in initial mobile phase. The samples undergo an ultracentrifugation step prior to injection onto the LC-MS/MS system and are analysed in a run time of 26 min. The LC-MS/MS system is run in MRM mode with both positive and negative electrospray ionisation. The method was validated over three days and is capable of quantitatively analysing for metronidazole, dimetridazole, ronidazole, ipronidazole, chloramphenicol, sulfamethazine, dinitolimide, ethopabate, carbadox and clopidol. The method is also capable of qualitatively analysing for sulfadiazine, tylosin, virginiamycin and avilamycin. A level of 100 microg kg(-1) was used for validation purposes and the method is capable of analysing to this level for all the compounds. Validation criteria of trueness, precision, repeatability and reproducibility along with measurement uncertainty are calculated for all analytes. |
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Authors:
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Mark Cronly; P Behan; B Foley; E Malone; S Earley; M Gallagher; P Shearan; L Regan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies Date: 2010-07-01 |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 53 ISSN: 1873-264X ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-08-16 Completed Date: 2010-12-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
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Languages: eng Pagination: 929-38 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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School of Pharmaceutical and Chemical Sciences, Dublin Institute of Technology, Kevin St., Dublin 8, Ireland. mark.cronly@statelab.ie |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed
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analysis* Animals Chromatography, Liquid / methods* Pharmaceutical Preparations / analysis* Poultry Reproducibility of Results Swine Tandem Mass Spectrometry / methods* |
| Chemical | |
Reg. No./Substance:
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0/Pharmaceutical Preparations |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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