| Development and validation of in vitro induction assays for toxic halogenated aromatic mixtures: a review. | |
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MedLine Citation:
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PMID: 2683222 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Halogenated aromatic industrial compounds, typified by the polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs) and biphenyls (PCBs) have been identified as residues in almost every component of the global ecosystem. Risk assessment of the complex mixtures of halogenated aromatics found in environmental samples is complicated by analytical problems and the lack of toxicological information on individual compounds and mixtures. Research in our laboratory has focused on the development and vadidation of the in vitro aryl hydrocarbon hydroxylase (AHH) induction assay in rat hepatoma H-4-II E cells in culture for quantitating individual toxic halogenated aryl hydrocarbons and their mixtures. For several PCB, PCDD, PCDF congeners, their mixed bromo/chloro analogs and reconstituted mixtures there was an excellent linear correlation between their -log ED50 values for AHH induction in rat hepatoma cells and their -log ED50 values for in vivo hepatic microsomal AHH induction, inhibition of body weight gain and thymic atrophy in the rat. It has also been shown for selected compounds that there was a good correlation between their in vitro AHH induction potencies and their effects in guinea pigs (AHH induction, inhibition of body weight gain) and mice (immunotoxicity). This assay system has been utilized to quantitative the "2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents" present in extracts from diverse sources including fly ash from a municipal incinerator and pyrolyzed brominated flame retardants which contain a complex mixture of halogenated dibenzo-p-dioxins and dibenzofurans. |
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Authors:
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S Safe; G Mason; T Sawyer; T Zacharewski; M Harris; C Yao; B Keys; K Farrell; M Holcomb; D Davis |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review |
Journal Detail:
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Title: Toxicology and industrial health Volume: 5 ISSN: 0748-2337 ISO Abbreviation: Toxicol Ind Health Publication Date: 1989 Oct |
Date Detail:
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Created Date: 1989-12-05 Completed Date: 1989-12-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8602702 Medline TA: Toxicol Ind Health Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 757-75 Citation Subset: IM |
Affiliation:
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Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A&M University, College Station 77843. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aryl Hydrocarbon Hydroxylases / biosynthesis* Cytochrome P-450 CYP1A1 Cytochrome P-450 Enzyme System / biosynthesis* Enzyme Induction / drug effects Hydrocarbons, Halogenated / toxicity* Oxidoreductases / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Hydrocarbons, Halogenated; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Oxidoreductases; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/Cytochrome P-450 CYP1A1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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