Document Detail

Development and validation of a high-performance liquid chromatography method for the simultaneous determination of aspirin and folic acid from nano-particulate systems.
MedLine Citation:
PMID:  20033881     Owner:  NLM     Status:  MEDLINE    
Attention has shifted from the treatment of colorectal cancer (CRC) to chemoprevention using aspirin and folic acid as agents capable of preventing the onset of colon cancer. However, no sensitive analytical method exists to simultaneously quantify the two drugs when released from polymer-based nanoparticles. Thus, a rapid, highly sensitive method of high-performance liquid chromatography analysis to simultaneously detect low quantities of aspirin (hydrolyzed to salicylic acid, the active moiety) and folic acid released from biodegradable polylactide-co-glycolide (PLGA) copolymer nanoparticles was developed. Analysis was done on a reversed-phase C(18) column using a photodiode array detector at wavelengths of 233 nm (salicylic acid) and 277 nm (folic acid). The mobile phase consisted of acetonitrile-0.1% trifluoroacetic acid mixture programmed for a 30 min gradient elution analysis. In the range of 0.1-100 microg/mL, the assay showed good linearity for salicylic acid (R(2) = 0.9996) and folic acid (R(2) = 0.9998). The method demonstrated good reproducibility, intra- and inter-day precision and accuracy (99.67, 100.1%) and low values of detection (0.03, 0.01 microg/mL) and quantitation (0.1 and 0.05 microg/mL) for salicylic acid and folic acid, respectively. The suitability of the method was demonstrated by simultaneously determining salicylic acid and folic acid released from PLGA nanoparticles.
Abhishek Chaudhary; Jeffrey Wang; Sunil Prabhu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  24     ISSN:  1099-0801     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-11     Completed Date:  2010-11-22     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  England    
Other Details:
Languages:  eng     Pagination:  919-25     Citation Subset:  IM    
Copyright Information:
2009 John Wiley & Sons, Ltd.
Department of Pharmaceutical Sciences, Western University of Health Sciences, Pomona, CA 91766, USA.
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MeSH Terms
Aspirin / analysis*
Chromatography, High Pressure Liquid / methods*
Folic Acid / analysis*
Lactic Acid / chemistry
Nanoparticles / chemistry*
Polyglycolic Acid / chemistry
Reproducibility of Results
Salicylic Acid / analysis
Grant Support
1R03CA121409-01/CA/NCI NIH HHS; R03 CA121409-01A1/CA/NCI NIH HHS
Reg. No./Substance:
0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 50-78-2/Aspirin; 59-30-3/Folic Acid; 69-72-7/Salicylic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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