| Development and validation of a high-performance liquid chromatography method for the simultaneous determination of aspirin and folic acid from nano-particulate systems. | |
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MedLine Citation:
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PMID: 20033881 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Attention has shifted from the treatment of colorectal cancer (CRC) to chemoprevention using aspirin and folic acid as agents capable of preventing the onset of colon cancer. However, no sensitive analytical method exists to simultaneously quantify the two drugs when released from polymer-based nanoparticles. Thus, a rapid, highly sensitive method of high-performance liquid chromatography analysis to simultaneously detect low quantities of aspirin (hydrolyzed to salicylic acid, the active moiety) and folic acid released from biodegradable polylactide-co-glycolide (PLGA) copolymer nanoparticles was developed. Analysis was done on a reversed-phase C(18) column using a photodiode array detector at wavelengths of 233 nm (salicylic acid) and 277 nm (folic acid). The mobile phase consisted of acetonitrile-0.1% trifluoroacetic acid mixture programmed for a 30 min gradient elution analysis. In the range of 0.1-100 microg/mL, the assay showed good linearity for salicylic acid (R(2) = 0.9996) and folic acid (R(2) = 0.9998). The method demonstrated good reproducibility, intra- and inter-day precision and accuracy (99.67, 100.1%) and low values of detection (0.03, 0.01 microg/mL) and quantitation (0.1 and 0.05 microg/mL) for salicylic acid and folic acid, respectively. The suitability of the method was demonstrated by simultaneously determining salicylic acid and folic acid released from PLGA nanoparticles. |
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Authors:
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Abhishek Chaudhary; Jeffrey Wang; Sunil Prabhu |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: 24 ISSN: 1099-0801 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-11 Completed Date: 2010-11-22 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: England |
Other Details:
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Languages: eng Pagination: 919-25 Citation Subset: IM |
Copyright Information:
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2009 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Pharmaceutical Sciences, Western University of Health Sciences, Pomona, CA 91766, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspirin
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analysis* Chromatography, High Pressure Liquid / methods* Folic Acid / analysis* Lactic Acid / chemistry Nanoparticles / chemistry* Polyglycolic Acid / chemistry Reproducibility of Results Salicylic Acid / analysis |
| Grant Support | |
ID/Acronym/Agency:
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1R03CA121409-01/CA/NCI NIH HHS; R03 CA121409-01A1/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 50-78-2/Aspirin; 59-30-3/Folic Acid; 69-72-7/Salicylic Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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