| Development and validation of flow cytometry methods for pharmacodynamic clinical biomarkers. | |
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MedLine Citation:
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PMID: 21083290 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Flow cytometry is a powerful analytical tool for the analysis of multiple biological parameters of individual cells or particles within heterogeneous cell populations. It has been widely used in biomedical research to perform immunophenotyping, cell counting and numerous cell function assessments, such as intracellular cytokine production, protein phosphorylation, cell proliferation and apoptosis. The implementation of standardized flow cytometry-based biomarker assays in clinical trials remains a challenge due to the limited stability of clinical specimens and the technical variations between instruments. To ensure data quality, it is crucial to develop robust assays for clinical applications. In this review, we summarize current practice in developing, validating and implementing flow cytometry assays to evaluate biomarkers in clinical research. |
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Authors:
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Dianna Y Wu; Lisa Patti-Diaz; Carla G Hill |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioanalysis Volume: 2 ISSN: 1757-6199 ISO Abbreviation: Bioanalysis Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-11-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101512484 Medline TA: Bioanalysis Country: England |
Other Details:
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Languages: eng Pagination: 1617-26 Citation Subset: IM |
Affiliation:
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Bristol-Myers Squibb Company, 311 Pennington-Rocky Hill Road, Mail Stop 3B-201, Pennington, NJ 08534, USA. dianna.wu@bms.com |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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