Document Detail

Development and validation of dried blood spots technique for quantitative determination of topiramate using liquid chromatography-tandem mass spectrometry.
MedLine Citation:
PMID:  23529887     Owner:  NLM     Status:  Publisher    
An LC-MS/MS method for determination of the anti-epileptic drug topiramate (TPM) in dried blood spot (DBS) samples was developed and validated. DBS samples were prepared by spotting 30 μL of spiked whole blood onto FTA(TM) DMPK-C cards and drying for at least 3 h. Six-millimetre punched spots were then extracted by using a mixture of methanol and water (90:10, v/v) with deuterated internal standard (topiramate-d12 ). The extracted samples were injected into a liquid chromatograph equipped with a tandem mass spectrometric detector. Negative ions were monitored in the selected reaction monitoring mode and transitions m/z 338.2 → 78.1 and m/z 350.3 → 78.1 were used for the quantitative evaluation of TPM and internal standard, respectively. The results obtained from validation were statistically evaluated according to the requirements of the European Medicines Agency and US Food and Drug Administration regulatory guidelines. The linearity of the method was checked within a concentration range from 10 to 2000 ng/mL. The validation results indicate that the method is accurate, precise, sensitive, selective and reproducible. Copyright © 2013 John Wiley & Sons, Ltd.
Tanja Vnučec Popov; Lea Cvitkovič Maričič; Helena Prosen; Darinka Brodnjak Vončina
Related Documents :
24492027 - Usefulness of measuring serum procalcitonin levels by immunochro-matographic assay in f...
23484787 - Dispersive liquid-liquid microextraction of propranolol enantiomers from human plasma b...
11489387 - A stability study of tetracycline and tetracycline cyclodextrins in tablets using a new...
23264717 - Detection of total microcystin in fish tissues based on lemieux oxidation, and recovery...
12353537 - Boiling point measurement of a small amount of brake fluid by thermocouple and its appl...
7451417 - Analysis of cholesterol in high density lipoprotein subfractions by high performance li...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-3-26
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  -     ISSN:  1099-0801     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-3-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 John Wiley & Sons, Ltd.
Krka, d.d., Novo mesto, Šmarješka cesta 6, SI-8501, Novo mesto, Slovenia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Obstructive Sleep Apnea Mimics Attention Deficit Disorder.
Next Document:  Determination of the Design Space of the HPLC Analysis of Water Soluble Vitamins.