Document Detail

Development, validation, and application of an acetylcholinesterase-biosensor test for the direct detection of insecticide residues in infant food.
MedLine Citation:
PMID:  12392961     Owner:  NLM     Status:  MEDLINE    
A highly sensitive and rapid food-screening test based on disposable screen-printed biosensors was developed, which is suitable for monitoring infant food. The exposure of infants and children to neurotoxic organophosphates and carbamates is of particular concern because of their higher susceptibility to adverse effects. The European Union has, therefore, set a very low limit for pesticides in infant food, which must not contain concentrations exceeding 10 microg/kg for any given pesticide. The maximum residue limit (MRL) has been set to be near the determination threshold that is typically achieved for pesticides with traditional analytical methods. The biosensor method could detect levels lower than 5 microg/kg and thus clearly fulfills the demands of the EU. To substantiate these measurements, recovery rates were determined and amounted on average to 104% in food. Matrix effects were eliminated by the introduction of a special electrode treatment. The test was compared with two traditional pesticide multiresidue analysis methods (GC-MS, LC-MS) using 26 fruit and vegetable samples from local markets and 23 samples of processed infant food from Germany, Spain, Poland and USA. Three infant food samples exceeded the MRL of 10 microg/kg when analyzed by either biosensor test or multiresidue methods.
Holger Schulze; Ellen Scherbaum; Michelangelo Anastassiades; Sandra Vorlová; Rolf D Schmid; Till T Bachmann
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  17     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-10-23     Completed Date:  2003-06-18     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1095-1105     Citation Subset:  IM    
Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany.
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MeSH Terms
Acetylcholinesterase / chemistry*
Biosensing Techniques / instrumentation*,  methods,  standards
Child, Preschool
Cholinesterase Inhibitors / analysis,  chemistry
Electrochemistry / instrumentation,  methods
Enzymes, Immobilized
Equipment Design
Equipment Failure Analysis
Food Contamination / analysis*
Infant Food / analysis*
Infant, Newborn
Insecticides / analysis*,  chemistry
Organophosphorus Compounds
Pesticide Residues / analysis,  standards*
Quality Control
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Carbamates; 0/Cholinesterase Inhibitors; 0/Enzymes, Immobilized; 0/Insecticides; 0/Organophosphorus Compounds; 0/Pesticide Residues; EC

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