| Development and validation of an HPLC-UV-visible method for sunitinib quantification in human plasma. | |
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MedLine Citation:
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PMID: 19341717 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Sunitinib malate is a novel oral multitargeted tyrosine kinase inhibitor with antitumor and antiangiogenic activities. Only mass spectrometry detection is currently available to determine sunitinib in human plasma. The purpose of this study was to develop a simple and sensitive high-performance liquid chromatographic method with UV-Visible detection for quantification of sunitinib concentrations in human plasma. METHODS: After a liquid-liquid extraction with ethyl acetate, sunitinib and ranitidine (internal standard) are separated on cyanopropyl column using a simple binary mobile phase of ammonium acetate buffer (20 mM; pH 6.8):acetonitrile (55:45,v/v). Samples were eluted isocratically at a flow rate of 1 mL/min throughout the 10 min run. Dual wavelength mode was used, with ranitidine monitored at 255 nm, and sunitinib at 431 nm. RESULTS: The calibration was linear in the range 20-200 ng/mL. Inter- and intra-day coefficients of variation were less than 7%. This method is sensitive, accurate and selective. It has been successfully implemented to monitor trough sunitinib concentrations in plasma samples (n = 39) from 14 unselected cancer patients treated with the recommended once daily dose of 50 mg or less. CONCLUSION: This method can be used in routine clinical practice to monitor plasma sunitinib concentrations in cancer patients treated with once daily administration. |
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Authors:
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Benoit Blanchet; Carole Saboureau; Anne Sophie Benichou; Bertrand Billemont; Fabrice Taieb; Stanislas Ropert; Alain Dauphin; François Goldwasser; Michel Tod |
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Publication Detail:
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Type: Journal Article; Validation Studies Date: 2009-03-31 |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 404 ISSN: 1873-3492 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-29 Completed Date: 2009-07-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 134-9 Citation Subset: IM |
Affiliation:
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Laboratoire de Pharmacologie-Toxicologie, Service de Pharmacie, GH Cochin-Saint Vincent-de-Paul, 75014 Paris, France. benoit.blanchet@cch.aphp.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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administration & dosage,
blood*,
therapeutic use Chromatography, High Pressure Liquid / methods* Drug Monitoring / methods* Humans Indoles / administration & dosage, blood*, therapeutic use Linear Models Neoplasms / blood*, drug therapy Protein Kinase Inhibitors / administration & dosage, blood*, therapeutic use Pyrroles / administration & dosage, blood*, therapeutic use Sensitivity and Specificity Spectrophotometry, Ultraviolet / methods* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Indoles; 0/Protein Kinase Inhibitors; 0/Pyrroles; 0/sunitinib |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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