Document Detail


Development and validation of an HPLC-UV-visible method for sunitinib quantification in human plasma.
MedLine Citation:
PMID:  19341717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Sunitinib malate is a novel oral multitargeted tyrosine kinase inhibitor with antitumor and antiangiogenic activities. Only mass spectrometry detection is currently available to determine sunitinib in human plasma. The purpose of this study was to develop a simple and sensitive high-performance liquid chromatographic method with UV-Visible detection for quantification of sunitinib concentrations in human plasma. METHODS: After a liquid-liquid extraction with ethyl acetate, sunitinib and ranitidine (internal standard) are separated on cyanopropyl column using a simple binary mobile phase of ammonium acetate buffer (20 mM; pH 6.8):acetonitrile (55:45,v/v). Samples were eluted isocratically at a flow rate of 1 mL/min throughout the 10 min run. Dual wavelength mode was used, with ranitidine monitored at 255 nm, and sunitinib at 431 nm. RESULTS: The calibration was linear in the range 20-200 ng/mL. Inter- and intra-day coefficients of variation were less than 7%. This method is sensitive, accurate and selective. It has been successfully implemented to monitor trough sunitinib concentrations in plasma samples (n = 39) from 14 unselected cancer patients treated with the recommended once daily dose of 50 mg or less. CONCLUSION: This method can be used in routine clinical practice to monitor plasma sunitinib concentrations in cancer patients treated with once daily administration.
Authors:
Benoit Blanchet; Carole Saboureau; Anne Sophie Benichou; Bertrand Billemont; Fabrice Taieb; Stanislas Ropert; Alain Dauphin; François Goldwasser; Michel Tod
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Publication Detail:
Type:  Journal Article; Validation Studies     Date:  2009-03-31
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  404     ISSN:  1873-3492     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-29     Completed Date:  2009-07-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  134-9     Citation Subset:  IM    
Affiliation:
Laboratoire de Pharmacologie-Toxicologie, Service de Pharmacie, GH Cochin-Saint Vincent-de-Paul, 75014 Paris, France. benoit.blanchet@cch.aphp.fr
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / administration & dosage,  blood*,  therapeutic use
Chromatography, High Pressure Liquid / methods*
Drug Monitoring / methods*
Humans
Indoles / administration & dosage,  blood*,  therapeutic use
Linear Models
Neoplasms / blood*,  drug therapy
Protein Kinase Inhibitors / administration & dosage,  blood*,  therapeutic use
Pyrroles / administration & dosage,  blood*,  therapeutic use
Sensitivity and Specificity
Spectrophotometry, Ultraviolet / methods*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Indoles; 0/Protein Kinase Inhibitors; 0/Pyrroles; 0/sunitinib

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