Document Detail


Development of transgenic rice plants overexpressing the Arabidopsis H+/Ca2+ antiporter CAX1 gene.
MedLine Citation:
PMID:  15372195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gene of the Arabidopsis thaliana H+/Ca2+ transporter, CAX1 (cation exchanger 1) was introduced into Japonica cultivars of rice (Ilpumbyeo) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice callus. The introduction of the CAX1 gene was also proven by PCR using CAX1-specific oligonucleotide primers in regenerated plants. Stable integration and expression of the CAX1 gene in T0 plants and T1 progeny were confirmed by DNA hybridization, Northern blot analysis, and luminescent analysis.
Authors:
K-M Kim; Y-H Park; C K Kim; K Hirschi; J-K Sohn
Related Documents :
19490895 - Proteasome-mediated turnover of the transcription coactivator npr1 plays dual roles in ...
10805595 - Post-transcriptional gene silencing in cultured rice cells.
17006595 - Characterization of the plant notchless homolog, a wd repeat protein involved in seed d...
22666345 - Transcriptomic characterization of temperature stress responses in larval zebrafish.
18956175 - A cation/proton-exchanging protein is a candidate for the barley necs1 gene controlling...
23438815 - Canonical terminal patterning is an evolutionary novelty.
Publication Detail:
Type:  Journal Article     Date:  2004-09-15
Journal Detail:
Title:  Plant cell reports     Volume:  23     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-04     Completed Date:  2005-06-16     Revised Date:  2006-09-27    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  678-82     Citation Subset:  IM    
Affiliation:
Institute of Genetic Engineering, Kyungpook National University, Daegu, 702-701, South Korea. kkm@knu.ac.kr
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antiporters / genetics*
Arabidopsis / genetics*
Arabidopsis Proteins / genetics*
Blotting, Southern
Calcium-Binding Proteins / genetics*
Cation Transport Proteins / genetics*
Gene Expression
Oryza sativa / genetics*
Plants, Genetically Modified / genetics*
Polymerase Chain Reaction
Recombinant Proteins / biosynthesis,  genetics
Tissue Culture Techniques
Transformation, Genetic*
Chemical
Reg. No./Substance:
0/Antiporters; 0/Arabidopsis Proteins; 0/Calcium-Binding Proteins; 0/Cation Transport Proteins; 0/Recombinant Proteins; 0/calcium-hydrogen antiporters

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Efficient developmental mis-targeting by the sporamin NTPP vacuolar signal to plastids in young leav...
Next Document:  Regeneration of pea (Pisum sativum L.) by a cyclic organogenic system.