Document Detail


Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique.
MedLine Citation:
PMID:  18035412     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is a clinical need for a tissue-engineered vascular graft (TEVG), and combining stem cells with biodegradable tubular scaffolds appears to be a promising approach. The goal of this study was to characterize the incorporation of muscle-derived stem cells (MDSCs) within tubular poly(ester urethane) urea (PEUU) scaffolds in vitro to understand their interaction, and to evaluate the mechanical properties of the constructs for vascular applications. Porous PEUU scaffolds were seeded with MDSCs using our recently described rotational vacuum seeding device, and cultured inside a spinner flask for 3 or 7 days. Cell viability, number, distribution and phenotype were assessed along with the suture retention strength and uniaxial mechanical behavior of the TEVGs. The seeding device allowed rapid even distribution of cells within the scaffolds. After 3 days, the constructs appeared completely populated with cells that were spread within the polymer. Cells underwent a population doubling of 2.1-fold, with a population doubling time of 35 h. Stem cell antigen-1 (Sca-1) expression by the cells remained high after 7 days in culture (77+/-20% vs. 66+/-6% at day 0) while CD34 expression was reduced (19+/-12% vs. 61+/-10% at day 0) and myosin heavy chain expression was scarce (not quantified). The estimated burst strength of the TEVG constructs was 2127+/-900 mm Hg and suture retention strength was 1.3+/-0.3N. We conclude from this study that MDSCs can be rapidly seeded within porous biodegradable tubular scaffolds while maintaining cell viability and high proliferation rates and without losing stem cell phenotype for up to 7 days of in-vitro culture. The successful integration of these steps is thought necessary to provide rapid availability of TEVGs, which is essential for clinical translation.
Authors:
Alejandro Nieponice; Lorenzo Soletti; Jianjun Guan; Bridget M Deasy; Johnny Huard; William R Wagner; David A Vorp
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-11-26
Journal Detail:
Title:  Biomaterials     Volume:  29     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2007-12-24     Completed Date:  2008-02-29     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  825-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials*
Blood Vessel Prosthesis*
Cell Separation
Cells, Cultured
Mice
Muscles / cytology*
Stem Cells / cytology*
Tissue Engineering / methods*
Grant Support
ID/Acronym/Agency:
R01 HL069368/HL/NHLBI NIH HHS; R01 HL069368/HL/NHLBI NIH HHS; R01 HL069368-05/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biocompatible Materials
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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