Document Detail

Development of a three-dimensional model for rapid evaluation of bone substitutes in vitro: effect of the 45S5 bioglass.
MedLine Citation:
PMID:  20540096     Owner:  NLM     Status:  MEDLINE    
The evaluation of innovative bone substitutes requires the development of an optimal model close to physiological conditions. An interesting alternative is the use of an immortalized cell line to construct multicellular spheroids, that is, three-dimensional (3D) cultures. In this study, a modified hanging drops method has resulted in the generation of spheroids with a well-established human fetal osteoblasts line (hFOB 1.19), and tests have been focused on the effect of 45S5 bioglass ionic dissolution products in comparison with two-dimensional (2D) cultures. Depending on cell culture type, quantitative analysis (cell proliferation, viability/cytotoxicity, and cellular cycle) and qualitative analysis (electron microscopy and genes expression) showed a differential effect. Cell proliferation was enhanced in 2D-conditioned cultures in accordance with literature data, but decreased in 3D cultures submitted to the same conditions, without change of gene expression patterns. The decrease of cell proliferation, observed in conditioned spheroids, appears to be in agreement with clinical observations showing the insufficiency of commercially available bioglasses for bone repairing within nonbearing sites, such as periodontal defects or small bone filling, in general. Therefore, we suggest that this model could be adapted to the screening of innovative bioactive materials by laboratory techniques already available and extended monitoring of their bioactivity.
Nora Alno; Franck Jegoux; Pascal Pellen-Mussi; Sylvie Tricot-Doleux; Hassane Oudadesse; Guy Cathelineau; Gilbert De Mello
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  95     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-08-26     Completed Date:  2010-12-08     Revised Date:  2012-05-04    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  137-45     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Service de Chirurgie et Pathologie Buccales, Centre Hospitalier Universitaire, Rennes, France.
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MeSH Terms
Biological Assay
Bone Substitutes / pharmacology*
Cell Cycle / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Ceramics / pharmacology*
Gene Expression Regulation / drug effects
Models, Biological*
Osteoblasts / cytology,  drug effects,  metabolism
Spheroids, Cellular / cytology,  drug effects,  ultrastructure
Reg. No./Substance:
0/Bone Substitutes; 0/Ceramics; 0/bioactive glass 45S5

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