Document Detail


Development of tailor-made glycidyl methacrylate-divinyl benzene copolymer for immobilization of D-amino acid oxidase from Aspergillus species strain 020 and its application in the bioconversion of cephalosporin C.
MedLine Citation:
PMID:  10704992     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A tailor-made glycidyl methacrylate-divinyl benzene (GMA-DVB) copolymer PC-3 was evolved by studying the effect of synthesis variables on binding and expression of D-amino acid oxidase (DAAO) from Aspergillus species strain 020. Almost quantitative binding (100%) and a high yield of immobilization per unit of enzyme loaded was achieved. Optimum pH, optimum temperature and K(m)95% was achieved by using 3% (w/v) solution of ceph C, 48 U of DAAO per g of ceph C, keeping dissolved oxygen level above 50%, maintaining the pH between 7.6 and 7.8 and temperature at 24 degrees C. The immobilized DAAO was used for 60 cycles in a stirred tank reactor.
Authors:
Mujawar; Kotha; Rajan; Ponrathnam; Shewale
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Journal of biotechnology     Volume:  75     ISSN:  1873-4863     ISO Abbreviation:  J. Biotechnol.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  2000-03-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  11-22     Citation Subset:  -    
Affiliation:
Research and Development, Hindustan Antibiotics Limited, Pimpri, Pune, India
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