Document Detail

Development of the steroidogenic capacity of medaka (Oryzias latipes) ovarian follicles during vitellogenesis and oocyte maturation.
MedLine Citation:
PMID:  3609704     Owner:  NLM     Status:  MEDLINE    
Developmental changes in the steroidogenic capacity of medaka, Oryzias latipes, ovarian follicles at 12 different stages during vitellogenesis and oocyte maturation were examined using 18-hr incubations. Medaka were acclimated to conditions of 26 degrees on a lighting regime of 14 hr light and 10 hr dark. Under these conditions, females usually spawn daily within 1 hr of the onset of light. The process of vitellogenesis and oocyte maturation occurs within 72 hr, the breakdown of the germinal vesicle (GVBD) and ovulation being completed at 6 and 1 hr, respectively, before the expected time of spawning. Vitellogenic follicles between 32 and 16 hr before spawning produced large amounts of estradiol-17 beta spontaneously and in response to partially purified chum salmon gonadotropin (SGA) or pregnant mare's serum gonadotropin (PMSG). However, postvitellogenic follicles between 12 and 4 hr before spawning showed very little evidence of estradiol-17 beta production. By contrast, basal concentrations of 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17 alpha,20 beta-diOHprog) remained very low in follicles during vitellogenesis and were elevated in those collected during oocyte maturation; there was a close relationship between the medium concentration of 17 alpha,20 beta-diOH-prog and the percentage GVBD in the oocytes. 17 alpha,20 beta-DiOHprog production in response to PMSG was very low in follicles during early and mid-vitellogenesis and increased in those collected at 28 hr before spawning, a time which coincided with the first acquisition of the ability of the follicles to undergo maturation in response to gonadotropin. These results clearly demonstrate that a distinct shift from the secretion of predominantly estradiol-17 beta to the secretion of 17 alpha,20 beta-diOHprog occurs in the medaka ovarian follicle immediately prior to oocyte maturation. Considering the potency of 17 alpha,20 beta-diOHprog for the induction of oocyte maturation in vitro, these results further suggest that 17 alpha,20 beta-diOHprog is a naturally occurring steroidal mediator of oocyte maturation in the medaka.
N Sakai; T Iwamatsu; K Yamauchi; Y Nagahama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  66     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-08-28     Completed Date:  1987-08-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  333-42     Citation Subset:  IM    
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MeSH Terms
Cyprinodontiformes / physiology*
Estradiol / secretion
Gonadotropins / pharmacology*
Gonadotropins, Equine / pharmacology
Hydroxyprogesterones / secretion
Oocytes / growth & development*
Oryzias / physiology*
Ovarian Follicle / drug effects,  physiology*
Steroids / secretion*
Time Factors
Vitellogenesis / drug effects*
Reg. No./Substance:
0/Gonadotropins; 0/Gonadotropins, Equine; 0/Hydroxyprogesterones; 0/Steroids; 0/salmon gonadotropin; 1662-06-2/17 alpha,20 beta-dihydroxypregn-4-en-3-one; 50-28-2/Estradiol

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