| Development of a solid-phase extraction/gas chromatographic-mass spectrometric method for quantification of succinic acid in nucleoside derivatives for oligonucleotide synthesis. | |
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MedLine Citation:
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PMID: 15861799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A solid-phase extraction (SPE)/gas chromatographic-mass spectrometric (GC-MS) method was developed for analysing residual succinic acid in nucleoside derivatives to be used in oligonucleotide synthesis. Use of a SPE protocol, enabled most of the derivatives to be trapped, thereby creating eluates enriched in succinic acid. GC-MS was used to quantify the amount of residual succinic acid in four different nucleoside preparations, with succinate concentrations varying from 0.18 to 0.24% (w/w). The within-day repeatability of the method was found to be 1.25% RSD. A linear relationship was observed between the amount of succinic acid in the sample and the GC-MS peak area, with a correlation coefficient of 0.9997 in the concentration interval 0.05-2.5% (w/w). Recoveries were measured by the addition of internal standards to working solutions and varied between 99.8 and 102.6%. |
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Authors:
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Ake Stenholm; Ingrid Drevin; Michael Lundgren |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1070 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-05-02 Completed Date: 2005-09-29 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 155-61 Citation Subset: IM |
Affiliation:
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Process Analytical Chemistry, Amersham Biosciences, Björkgatan 30, S-75184 Uppsala, Sweden. ake.stenholm@amersham.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Liquid Gas Chromatography-Mass Spectrometry / methods* Nucleosides / chemistry* Oligonucleotides / chemical synthesis* Reproducibility of Results Spectrophotometry, Ultraviolet Succinic Acid / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Nucleosides; 0/Oligonucleotides; 110-15-6/Succinic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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