Document Detail


Development of a solid-phase extraction/gas chromatographic-mass spectrometric method for quantification of succinic acid in nucleoside derivatives for oligonucleotide synthesis.
MedLine Citation:
PMID:  15861799     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A solid-phase extraction (SPE)/gas chromatographic-mass spectrometric (GC-MS) method was developed for analysing residual succinic acid in nucleoside derivatives to be used in oligonucleotide synthesis. Use of a SPE protocol, enabled most of the derivatives to be trapped, thereby creating eluates enriched in succinic acid. GC-MS was used to quantify the amount of residual succinic acid in four different nucleoside preparations, with succinate concentrations varying from 0.18 to 0.24% (w/w). The within-day repeatability of the method was found to be 1.25% RSD. A linear relationship was observed between the amount of succinic acid in the sample and the GC-MS peak area, with a correlation coefficient of 0.9997 in the concentration interval 0.05-2.5% (w/w). Recoveries were measured by the addition of internal standards to working solutions and varied between 99.8 and 102.6%.
Authors:
Ake Stenholm; Ingrid Drevin; Michael Lundgren
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1070     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-05-02     Completed Date:  2005-09-29     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  155-61     Citation Subset:  IM    
Affiliation:
Process Analytical Chemistry, Amersham Biosciences, Björkgatan 30, S-75184 Uppsala, Sweden. ake.stenholm@amersham.com
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Liquid
Gas Chromatography-Mass Spectrometry / methods*
Nucleosides / chemistry*
Oligonucleotides / chemical synthesis*
Reproducibility of Results
Spectrophotometry, Ultraviolet
Succinic Acid / analysis*
Chemical
Reg. No./Substance:
0/Nucleosides; 0/Oligonucleotides; 110-15-6/Succinic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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