| Development of a single-pass ceramic matrix bioreactor for large-scale mammalian cell culture. | |
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MedLine Citation:
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PMID: 18601214 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A single-pass, plug-flow bioreactor has been developed in which oxygen is supplied to entrapped hybridoma cells via sllicone tubes threaded through the square channels of a macroporous ceramic monolith. Oxygen diffuses from the gas phase, through the silicone tubing, across the open square channel, and into the pores of the ceramic wall where it is consumed by entrapped cells. Advantages of such a reactor include higher product yields, protection of cells from detrimental hydrodynamic effects, no internal moving parts to compromise asepsis, and simplicity of operation. A prototype bioreactor was constructed and operated over a range of residence times. A side-by-side experimental comparison with a conventional recycle bioreactor was performed by inoculating both bioreactors with cells from the same stock culture and feeding medium from the same reservoir. Final antibody titers were 80% higher in the single-pass bioreactor at a residence time of 200 minutes compared with those of the recycle bioreactor at a residence time of 800 minutes. A theoretical analysis of oxygen transport in this bioreactor is developed to highlight important design criteria and operating strategies for scale-up. (c) 1992 John Wiley & Sons, Inc. |
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Authors:
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M A Applegate; G Stephanopoulos |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 40 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1992 Nov |
Date Detail:
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Created Date: 2008-07-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 1056-68 Citation Subset: - |
Affiliation:
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Department of Chemical Engineering, Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Cambridge, MA 02139. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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