| Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells. | |
| | |
MedLine Citation:
|
PMID: 18502895 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The recently developed technique of establishing embryonic stem (ES) cell lines from single blastomeres (BTMs) of early mouse and human embryos has created significant interest in this source of ES cells. However, sister BTMs of an early embryo might not have equal competence for the development of different lineages or the derivation of ES cells. Therefore, single BTMs from two- and four-cell embryos of outbred mice were individually placed in sequential cultures to enhance the formation of the inner cell mass (ICM) and the establishment of embryonic outgrowth. The outgrowths were then used for the derivation of ES cell lines. Based on the expression of ICM (Sox2) and trophectoderm (Cdx2) markers, it was determined that ICM marker was lacking in blastocysts derived from 12% of BTMs from two-cell stage and 20% from four-cell stage. Four ES cell lines (5.6%; 4/72) were established ater culture of single BTMs from two-cell embryos, and their pluripotency was demonstrated by their differentiation into neuronal cell types. Our results demonstrate that sister BTMs of an early embryo are not equally competent for ICM marker expression. However, we demonstrated the feasibility of establishing ES cells from a single BTM of outbred mice. |
| | |
Authors:
|
Chanchao Lorthongpanich; Shang-Hsun Yang; Karolina Piotrowska-Nitsche; Rangsun Parnpai; Anthony W S Chan |
Related Documents
:
|
11566725 - Genetic and epigenetic factors affecting blastomere fragmentation in two-cell stage mou... 15955835 - Variable reprogramming of the pluripotent stem cell marker oct4 in mouse clones: distin... 94085 - Cell differentiation in isolated inner cell masses of mouse blastocysts in vitro: onset... 11076765 - Taube nuss is a novel gene essential for the survival of pluripotent cells of early mou... 620005 - Extracellular hydrolases of the lung. 9094975 - Isolation and characterization of liver epithelial cell lines from wild-type and mutant... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Reproduction (Cambridge, England) Volume: 135 ISSN: 1741-7899 ISO Abbreviation: Reproduction Publication Date: 2008 Jun |
Date Detail:
|
Created Date: 2008-05-26 Completed Date: 2008-12-22 Revised Date: 2009-01-20 |
Medline Journal Info:
|
Nlm Unique ID: 100966036 Medline TA: Reproduction Country: England |
Other Details:
|
Languages: eng Pagination: 805-13 Citation Subset: IM |
Affiliation:
|
Yerkes National Primate Research Center, Department of Human Genetics and Genetics and Molecular Biology Program, Emory University School of Medicine, North East Atlanta, Georgia 30329, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Markers / analysis Blastocyst / cytology* Blastocyst Inner Cell Mass / cytology Blastomeres / cytology* Cell Count Cell Culture Techniques Cell Differentiation Cells, Cultured Cleavage Stage, Ovum Embryonic Stem Cells / cytology* Female Homeodomain Proteins / analysis Immunohistochemistry Mice SOXB1 Transcription Factors / analysis Vimentin / analysis |
| Grant Support | |
ID/Acronym/Agency:
|
RR-00165/RR/NCRR NIH HHS; RR018827-04/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 0/CDX2 protein, human; 0/Homeodomain Proteins; 0/SOXB1 Transcription Factors; 0/Sox2 protein, mouse; 0/Vimentin |
| Comments/Corrections | |
Erratum In:
|
Reproduction. 2008 Aug;136(2):275 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A mitochondrial mechanism is involved in apoptosis of Robertsonian mouse male germ cells.
Next Document: Developmental regulation of histone H3 methylation at lysine 4 in the porcine ovary.