Document Detail

Development of a simple, portable carbon dioxide incubator for in vitro production of bovine embryos.
MedLine Citation:
PMID:  10066102     Owner:  NLM     Status:  MEDLINE    
The objective of this study was to develop a simple and portable CO2 incubator using effervescent granules (EG) and to examine the effect of negative and positive air pressure for in vitro maturation (IVM), fertilization (IVF) and culture (IVC) of bovine oocytes. In experiment 1, cumulus-oocyte complexes (COCs) were matured (22 h), fertilized (5 h) and cultured (7 days) using 0.25, 0.5 or 1.0 g of EG per 0.6 l added to maintain an optimum level of CO2 (approximately 3, 6 or 12%, respectively) for in vitro production of embryos. Control oocytes, zygotes and embryos were cultured in a standard CO2 incubator. The blastocyst production rates observed on Days 7 to 9 after insemination were 20.5+/-4.2%, 18.5+/-3.9% and 28.7+/-5.1% for the 0.25 g EG, 0.5 g EG treatments and control, respectively. These rates were significantly higher (P < 0.05) than that of the 1.0 g EG treatment (8.7+/-2.6%). The number of cells in the inner cell mass (ICM) and trophectoderm (TE) produced from blastocysts using the control procedure were 40.8+/-2.9 and 81.2+/-5.3, respectively, and were higher (P < 0.05) compared to the 0.50 g EG (34.6+/-2.9 and 66.8+/-5.7) and 1.0 g EG treatments (33.4+/-3.4 and 67.2+/-7.3). In experiment 2, COCs were placed in a small box with 0.25 g of EG so that the effects on IVM, IVF and IVC of positive or negative air pressure could be compared. The blastocyst production rate observed in the negative air pressure treatment (29.6+/-4.6%) was higher (P < 0.01) than that of the positive air pressure treatment (6.2+/-1.5%) or the normal treatment pressure (P < 0.05; 18.7+/-4.2%) but did not differ from that of the control (30.7+/-4.4%). These results indicate that this simple type of incubator with negative air pressure can be successfully used for in vitro production of bovine embryos and could be used at the field level.
T Suzuki; C Sumantri; N H Khan; M Murakami; S Saha
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Animal reproduction science     Volume:  54     ISSN:  0378-4320     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-05-18     Completed Date:  1999-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  149-57     Citation Subset:  IM    
United Graduate School of Veterinary Sciences, Yamaguchi University, Japan.
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MeSH Terms
Air Pressure
Carbon Dioxide / physiology
Cattle / embryology*
Embryonic and Fetal Development / physiology*
Fertilization in Vitro / veterinary
Incubators / veterinary*
Oocytes / physiology
Sperm-Ovum Interactions / physiology
Spermatozoa / physiology
Reg. No./Substance:
124-38-9/Carbon Dioxide

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