| Development of a sensitive and rapid nucleic acid assay with tetraphenyl porphyrinatoiron chloride by a resonance light scattering technique. | |
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MedLine Citation:
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PMID: 17610304 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Based on the interaction between nucleic acids and tetraphenyl porphyrinatoiron chloride (FeTPPCl), a novel method for the determination of nucleic acids at the nanogram level has been developed. Under the optimum conditions, the weak resonance light scattering (RLS) intensity of FeTPPCl was greatly enhanced by nucleic acids and the enhanced RLS intensity was proportional to the concentration of nucleic acids in the range 0.02-2.8 microg/mL for calf thymus DNA, 0.05-3.3 microg/mL for fish sperm DNA and 0.07-4.5 microg/mL for yeast RNA. The detection limits (3sigma) were 2.9 ng/mL for calf thymus DNA, 3.9 ng/mL for fish sperm DNA and 5.2 ng/mL for yeast RNA. Almost no interference could be observed from proteins, nucleosides and most of the metal ions. The proposed method showed good reliability, sensitivity, rapidity and reproducibility when applied to the determination of nucleic acids in synthetic and biochemical samples. The time savings make this method suitable for large routine analyses. |
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Authors:
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Zhanguang Chen; Jinbin Liu; Li Zhu; Weifeng Ding; Yali Han |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Luminescence : the journal of biological and chemical luminescence Volume: 22 ISSN: 1522-7235 ISO Abbreviation: Luminescence Publication Date: 2007 Sep-Oct |
Date Detail:
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Created Date: 2007-10-02 Completed Date: 2007-12-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100889025 Medline TA: Luminescence Country: England |
Other Details:
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Languages: eng Pagination: 493-500 Citation Subset: IM |
Copyright Information:
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Copyright 2007 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Chemistry, Shantou University, Shantou 515063, People's Republic of China. kqlu@stu.edu.cn |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calibration Cattle DNA / analysis* Fishes Humans Hydrogen-Ion Concentration Ions / chemistry Light* Male Metalloporphyrins / chemistry* Metals / chemistry Molecular Structure RNA / analysis* Saccharomyces cerevisiae / chemistry Scattering, Radiation Sensitivity and Specificity Serum Albumin / chemistry Spermatozoa / chemistry Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Ions; 0/Metalloporphyrins; 0/Metals; 0/Serum Albumin; 0/tetraphenyl porphyrinatoiron chloride; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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