Document Detail


Development of a sensitive microtitration plate enzyme-immunoassay for the anabolic steroid trenbolone.
MedLine Citation:
PMID:  3595922     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of a competitive microtitration plate enzyme-immunoassay for monitoring trenbolone application to animals is described. 'Bridge heterology' was achieved with a rabbit antibody raised against 17 beta-trenbolone-hemisuccinate-BSA and 17 alpha-trenbolone glucuronide-alkaline phosphatase as a tracer. The required 17 alpha-trenbolone glucuronide was prepared by application of trenbolone acetate to a calf following isolation from urine by three HPLC steps. The glucuronide was linked to alkaline phosphatase by the mixed anhydride procedure. The EIA was performed by coating affinity purified sheep IgG antirabbit IgG to the microtitration plate well followed by the addition of sample, tracer and hormone-specific antibody. The absolute detection limit was less than 1 pg; 50% relative binding at approximately 3 pg which is about 20 times superior to our RIA. The sample blanks of purified extracts from urine, bile, faeces, liver and muscle were similar in both methods (EIA and RIA) and much lower than required for a reliable detection of positive samples. Assay variations were always less than 15%. For the EIA, radiolabelled trenbolone, which is not commercially available, is not necessary and according to our experience the EIA is more practicable and economic.
Authors:
H H Meyer; S Hoffmann
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Food additives and contaminants     Volume:  4     ISSN:  0265-203X     ISO Abbreviation:  Food Addit Contam     Publication Date:    1987 Apr-Jun
Date Detail:
Created Date:  1987-07-24     Completed Date:  1987-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500474     Medline TA:  Food Addit Contam     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  149-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase
Animals
Bile / analysis
Cattle
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay
Estrenes / analysis*
Feces / analysis
Liver / analysis
Muscles / analysis
Radioimmunoassay / methods
Trenbolone / analogs & derivatives,  analysis*
Chemical
Reg. No./Substance:
0/Estrenes; 0/trenbolone-17-glucuronide; 10161-33-8/Trenbolone; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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