Document Detail


Development of a semi-defined medium supporting production of platensimycin and platencin by Streptomyces platensis.
MedLine Citation:
PMID:  23188381     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Platensimycin and platencin are compounds that were discovered at Merck Research Laboratories and have shown promising antibacterial activity. They are both produced in fermentation by the actinomycete Streptomyces platensis. Merck reported a crude, insoluble production medium to produce the antibiotics. To test the possible effects of different primary metabolites and inorganic compounds on the production of these antibiotics, a chemically-defined medium is needed. The effects that these compounds have on production could provide information about the precursors and biosynthetic pathway of the antibiotics. We have tested and developed a number of media with varying degrees of chemical definition and solubility using the Merck medium as our starting point. Our latest production medium, PM5, is soluble and semi-defined. It yields suitable production of the compounds, as shown by agar diffusion assays, bioautography and HPLC. The antibiotics were located in the extracellular broths and not in the mycelia.The Journal of Antibiotics advance online publication, 28 November 2012; doi:10.1038/ja.2012.97.
Authors:
Sabrina Aluotto; Heather Tynan; Christian Maggio; Maria Falzone; Anisha Mukherjee; Vincent Gullo; Arnold L Demain
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-28
Journal Detail:
Title:  The Journal of antibiotics     Volume:  -     ISSN:  0021-8820     ISO Abbreviation:  J. Antibiot.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151115     Medline TA:  J Antibiot (Tokyo)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Charles A Dana Research Institute for Scientists Emerti (RISE), Drew University, Madison, NJ, USA.
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