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Development of a screening method for biogenic amine producing Bacillus spp.
MedLine Citation:
PMID:  22177850     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Most of the currently available screening methods for biogenic amine (BA)-producing microorganisms are based on differential media containing a pH indicator; BA production is detected as an increase in pH. However, such acid-alkaline detection methods cannot be adapted directly for use with alkaline bacteria such as Bacillus species. Here we describe an easy, rapid and reliable method for detecting BA-producing Bacillus strains using plate and broth methods. Positive selection using the plate method was achieved within 24h. The broth method proved more precise and enabled quantitation of the BA production, and correlated well with the plate method. False-negatives were diminished using the broth method, and false-positives could be screened out by an additional test for glycerol metabolism. Of the 12 strains examined using new method, five (B. licheniformis cy2, B. polyfermenticus CJ9, B. licheniformis KCTC1918, B. subtilis KCTC1028, and B. subtilis KCTC1022) were BA-positive with the broth method, among which KCTC1022 was determined to be a false-positive using the glycerol metabolism examination. The suitability and accuracy of the broth method was confirmed by high-performance liquid chromatography detection of BA formation.
Authors:
Mi Chang; Hae Choon Chang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-20
Journal Detail:
Title:  International journal of food microbiology     Volume:  -     ISSN:  1879-3460     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-12-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
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